胶原酶与不同药物相配伍对其酶活性影响的研究 |
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Received:March 14, 2006
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作者 | Author | 单位 | Unit | E-Mail |
金星 |
JIN Xing |
沈阳市骨科医院,辽宁沈阳110044 |
Shenyang Orthopaedic Hospital,Shenyang 110044,Liaoning,China |
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杨春生 |
YANG Chun-sheng |
沈阳市骨科医院 辽宁沈阳110044 |
Shenyang Orthopaedic Hospital,Shenyang 110044,Liaoning,China |
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章凤兰 |
ZHANG Feng-lan |
沈阳市骨科医院 辽宁沈阳110044 |
Shenyang Orthopaedic Hospital,Shenyang 110044,Liaoning,China |
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刘旸 |
LIU Yang |
沈阳市骨科医院 辽宁沈阳110044 |
Shenyang Orthopaedic Hospital,Shenyang 110044,Liaoning,China |
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毛碧峰 |
MAO Bi-Feng |
沈阳市骨科医院 辽宁沈阳110044 |
Shenyang Orthopaedic Hospital,Shenyang 110044,Liaoning,China |
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期刊信息:《中国骨伤》2007年20卷,第2期,第108-109页 |
DOI:doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
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目的:观察注射用胶原酶与不同药物相配伍后酶活性变化的情况,探讨胶原酶与不同药物配伍对酶活性的影响。
方法:将注射用胶原酶制剂与12种药物分别配伍后,将样品管、底物对照管和酶对照管各加入无水乙醇0.5ml终止酶反应,冷却过滤。取滤液稀释后置于TU-1800pc紫外可见分光光度计,将样品管的吸收度减去底物对照管和酶对照管的吸收度,再从标准曲线上查得亮氨酸的μg数,以胶原酶在37℃、pH7.5条件下作用于胶原蛋白,每小时从胶原蛋白中释放的多肽相当于茚三酮显色1μmol亮氨酸量为一个活性单位来计算注射用胶原酶制剂的活性。
结果:12种药物对注射用胶原酶的活性均有不同程度的影响,轻者导致胶原酶的活性下降,重者导致胶原酶的活性丧失。
结论:胶原酶的化学本质是蛋白质,任何能改变蛋白质性质的条件,都会使酶的活性下降或失活。忽视胶原酶的化学本质而随意配伍的不规范做法,有悖于酶促反应动力学的基本原理,在临床工作中不应提倡。 |
[关键词]:胶原酶类 配伍 酶活性 椎间盘化学松解术 |
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Research on activity effect of collagenase’s compatibility with different drugs |
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Abstract:
Objective: To observe the change of the activity of collagenase’s compatibility with different drugs.
Methods:The collagenase was mixed with twelve kinds of drugs.Adding anhydrous alcohol 0.5 ml to the sample tube,the subtrate control tube and the enzyme control tube respectively to stop enzyme reaction,cooling and filtering.Putting filtered solution which was diluted into TU-1800PC type ultraviolet photometer,subtracting the absorption ratio of the subtrate control tube and the absorption ratio of the enzyme control tube from that of the example tube,checking leucine’s μg value from the standard curve.On the condition of 37 ℃ pH7.5,collagenase effected on collagen.Released peptide from collagen per hour was equivalent to 1 μmol leucine quantity from ninhydrin color reaction as one unit to calculate collagenase solution preparation’s activity.
Results: Twelve kinds of drugs had different degree effect on the collagenase’s activity.It could cause collagenase’s activity to decline in slight condition and loss in severe condition.
Conclusion:Collagenase is protein in chemical nature.Any conditions which change protein’s character can lead to enzyme’s activity declined or lost.This kind of un-standard way which ignore enzyme’s chemical nature and compatibility will violate enzymatic reaction dynamic’s basic theory,shouldn’t be advocated in clinical practice. |
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引用本文,请按以下格式著录参考文献: |
中文格式: | 金星,杨春生,章凤兰,刘旸,毛碧峰.胶原酶与不同药物相配伍对其酶活性影响的研究[J].中国骨伤,2007,20(2):108~109 |
英文格式: | JIN Xing,YANG Chun-sheng,ZHANG Feng-lan,LIU Yang,MAO Bi-Feng.Research on activity effect of collagenase’s compatibility with different drugs[J].zhongguo gu shang / China J Orthop Trauma ,2007,20(2):108~109 |
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