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膝骨关节炎滑膜细胞体外培养及生物学特性观察

Hits: 1951 Download times: 1789 Received:July 10, 2006

作者	Author	单位	Unit	E-Mail
张鹏	ZHANG Peng	上海市第六人民医院针推伤科,上海200233	Department of Traumatology and Orthopaedics of the 6th Hospital of Shanghai,Shanghai 200233,China	gtshi@sina.com
石关桐	SHI Guan-tong	上海中医药大学附属曙光医院骨伤科
郑昱新	ZHENG Yu-xin	上海中医药大学附属曙光医院骨伤科

期刊信息：《中国骨伤》2006年19卷，第11期，第656-658页

DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz

基金项目:国家自然科学基金资助项目(30472222)

目的:观察原发性膝骨关节炎患者滑膜细胞的体外生长特性,探讨骨性关节炎的发病机制。

方法:组织块培养法分离早期与晚期(依据Kellgren和Lawrence的放射学诊断标准划分)OA患者滑膜细胞进行培养,用光镜、电镜、组织化学

方法:等观察鉴定该细胞类型。

结果:患者滑膜细胞符合巨噬样滑膜细胞(A型细胞)及成纤维细胞样滑膜细胞(B型细胞)的特征,早期与晚期滑膜细胞在生长周期及细胞超微结构等方面存在差异。晚期较早期滑膜细胞生长周期长、生长极向性差、细胞内溶酶体丰富。

结论:组织块培养法可以有效分离人类关节滑膜细胞,建立原发性膝骨关节炎患者滑膜细胞系。

[关键词]:滑膜细胞,培养的骨关节炎,膝

Biologic characteristics of human synoviocytes of gonarthritis in vitro

Abstract:

Objective:To investigate the biological characteristics of human synoviocytes of primary gonarthritis in vitro.

Methods:Human synoviocytes were isolated from synovial membrane(earlier stage and later stage of OA) by tissue culture isolation,which were identified by light microscope,electro microscope,histochemical method.

Results:The isolated cells were in acordance with the characteristics of macrophage-like synoviocytes(type-A synoviocytes) and fibroblast-like synoviocytes (type-B synoviocytes).Differences showed in the growth cycles,differentiations and ultra microstructures between the two stages.

Conclusion:Tissue culture isolation is an effective isolation of human synoviocytes to establish the human synoviocyte line of primary gonarthritis.

KEYWORDS:Synovial membrane Cells,cultured Osteoarthritis,knee

引用本文，请按以下格式著录参考文献：

中文格式:	张鹏,石关桐,郑昱新.膝骨关节炎滑膜细胞体外培养及生物学特性观察[J].中国骨伤,2006,19(11):656~658
英文格式:	ZHANG Peng,SHI Guan-tong,ZHENG Yu-xin.Biologic characteristics of human synoviocytes of gonarthritis in vitro[J].zhongguo gu shang / China J Orthop Trauma ,2006,19(11):656~658

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