| 益气化瘀方对椎间盘软骨细胞凋亡相关因子的作用 |
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Received:May 16, 2006
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| 期刊信息：《中国骨伤》2006年19卷，第8期，第449-451页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:国家自然科学基金项目(编号:30171170&30572398);国家自然科学基金重点项目(编号:0330700);上海市科技“启明星”跟踪计划(编号:05QMH1412);上海市医学重点学科建设项目(编号:05Ⅲ027);上海市重点学科建设项目(编号:T0303) |
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目的:探讨益气化瘀方对椎间盘软骨细胞凋亡状态下Bax、Bcl-2及Caspase-8表达的调控作用。
方法:清洁级雄性1月龄SD大鼠20只,酶消化法获取椎间盘软骨细胞,用抗Fas抗体诱导其发生凋亡,通过益气化瘀方及其拆方益气方、化瘀方药理血清干预,免疫组化法(SP)检测分析大鼠椎间盘软骨细胞不同状态下Bax、Bcl-2及Caspase-8的表达。
结果:相对正常组,凋亡模型组的Bax和Caspase-8表达明显,而Bcl-2轻度表达,有显著差异(P<0.01)。益气化瘀方、益气方和化瘀方均能降低椎间盘软骨细胞的Bax、Caspase-8的表达,而上调Bcl-2的表达,3种中药组与诱导凋亡组相比有显著差异(P<0.01)。
结论:中药益气化瘀方、益气方和化瘀方都可以调控凋亡的大鼠椎间盘软骨细胞内Bax、Bcl-2、Caspase-8的表达,其治疗颈椎病的机制与其对凋亡相关因子的调控有关。 |
| [关键词]:椎间盘 软骨细胞 细胞凋亡 益气化瘀方 |
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| Effects of Yiqi-Huayu recipe (益气化瘀方) and its decomposed formulas on apoptosis-related factors of discus intervertebrale chondrocytes in rats |
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Abstract:
Objective: To study the effects of Yiqi-Huayu recipe (益气化瘀方) and its decomposed formulas-medicated on expressions of Bcl-2, Bax and Caspase-8 of apoptotic discus intervertebrale chondrocytes in rats.
Methods: Immunohistochemical and integral optical density analytic methods were used to observe the effects of Yiqi-Huayu recipe, Yiqi recipe, Huayu recipe-medicated sera on the expressions of Bcl-2, Bax and Caspase-8 of apoptotic discus intervertebrale chondrocytes in rats induced by anti-Fas antibody.
Results: As compared with apoptosis group, Bcl-2 expression was higher, Bax and Caspase-8 expressions were lower in Yiqi-Huayu-treated, Yiqi-treated, Huayu-treated (P<0.01).
Conclusion: Yiqi-Huayu recipe and its decomposed formulas can delay degeneration of the cervical intervertebral disc, which may be due to its action in regulating the expressions of Bcl-2, Bax and Caspase-8. |
| KEYWORDS:Intervertebral disc Chondrocytes Apoptosis Yiqi-Huayu recipe |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 孙鹏,王拥军,施杞,周泉,刘梅,周重建,钱雪华.益气化瘀方对椎间盘软骨细胞凋亡相关因子的作用[J].中国骨伤,2006,19(8):449~451 |
| 英文格式: | SUN Peng,WANG Yong-jun,SHI Qi,ZHOU Quan,LIU Mei,ZHOU Chong-jian,QIAN Xue-hua.Effects of Yiqi-Huayu recipe (益气化瘀方) and its decomposed formulas on apoptosis-related factors of discus intervertebrale chondrocytes in rats[J].zhongguo gu shang / China J Orthop Trauma ,2006,19(8):449~451 |
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