| 辛伐他汀抑制甲状旁腺素相关肽诱导的破骨细胞生成和功能 |
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Received:April 20, 2006
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| 作者 | Author | 单位 | Unit | E-Mail |
| 黄鲁豫 |
HUANG Luyu |
第四军医大学附属西京医院骨科研究所,陕西西安710032 |
Institute of Orthopaedics and Traumatology,Xijing Hospital,the Forth Military Medical University,Xi’an 710032,Shanxi,China |
hw en6407@ fmmu.ede.cn |
| 胡蕴玉 |
HU Yun-yu |
第四军医大学附属西京医院骨科研究所,陕西西安710032 |
Institute of Orthopaedics and Traumatology,Xijing Hospital,the Forth Military Medical University,Xi’an 710032,Shanxi,China |
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| 马真胜 |
LV Rong |
第四军医大学附属西京医院骨科研究所,陕西西安710032 |
Institute of Orthopaedics and Traumatology,Xijing Hospital,the Forth Military Medical University,Xi’an 710032,Shanxi,China |
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| 吕荣 |
HUANG Luyu |
第四军医大学附属西京医院骨科研究所,陕西西安710032 |
Institute of Orthopaedics and Traumatology,Xijing Hospital,the Forth Military Medical University,Xi’an 710032,Shanxi,China |
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| 期刊信息：《中国骨伤》2006年19卷，第7期，第420-423页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:陕西省科技攻关项目资助课题[编号:2005K14-G2(1)] |
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目的:探讨辛伐他汀对骨髓来源的破骨细胞形成和功能的影响。
方法:取Balb/C雄性小鼠双侧股骨和胫骨的骨髓,以不含血清的α-MEM培养液洗涤并收集骨髓细胞,再将细胞重新悬浮于含100ml/L胎牛血清的α-MEM培养液中,细胞计数后,配成1.5×109/L的细胞悬液,同时加入甲状旁腺素相关肽(PTHrP)和不同剂量的辛伐他汀(10-7、10-6、10-5mol/L)于24孔培养板进行培养,并设阳性对照(只加PTHrP)和阴性对照(PTHrP和辛伐他汀都不加)组,每组均有1孔放置骨磨片1片,培养6d后;去除上清,抗酒石酸(TRAP)染色检测培养板底部破骨细胞形成;骨磨片用甲苯胺蓝染色,电镜检测骨磨片的吸收陷窝。
结果:小鼠骨髓细胞在PTHrP的诱导下获得大量的TRAP染色阳性的破骨细胞,骨磨片有吸收陷窝形成;用辛伐他汀(10-7、10-6mol/L)和PTHrP共同培养下TRAP染色阳性的破骨细胞形成数量均明显减少(P<0.01),辛伐他汀在10-5mol/L时则无TRAP染色阳性的破骨细胞形成;辛伐他汀在10-7mol/L时骨磨片有吸收陷窝的形成但少于阳性对照组(P<0.01),在10-6、10-5mol/L时骨磨片则无吸收陷窝的形成。
结论:辛伐他汀对小鼠骨髓来源的破骨细胞的形成有着明显的抑制作用,并且呈剂量依赖关系。 |
| [关键词]:破骨细胞 辛伐他汀 细胞培养 |
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| Inhibition of simvastatin on osteoclast formation and function induced by PTHrP |
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Abstract:
Objective:To evaluate the effect of simvastatin on the osteoclast formation and function by source of bone marrow.
Methods:Bilateral femoral and tibial bone marrow of Bale/C male mice were taken and cultured with α-MEM nutrient solution in which no serum was contained.After washing,murine primary bone marrow cells were gathered and re-suspended in the α-MEM nutrient solution with 100 ml/L fetal bovine serum.The cell suspension in 1.5×109/L concentration was made after counting.Under the induction by PTHrP,the cells were cultured for 6 days in the presence of 10-7,10-6,10-5 mol/L smivastatin.Meanwhile,the positive and negative control group was set up.Osteoclast formation was detected by TRAP staining and its function was assessed by absorption lacuna using electron microscope.
Results:Large osteoclasts and absorption lacuna were formed under the induction of PTHrP.Osteoclast formation were partially inhibited adding in 10-7,10-6 mol/L simvastatin（P<0.01）,and almost no positive osteoclast formed adding in 10-5 mol/L simvastatin.The absorption lacuna was partially fewer adding in(10-7 mol/L) simvastatin than that of positive group（P<0.01）,and no absorption lacuna was found at the high dose of simvastain(106,10-5 mol/L).Conclusions:Simvastatin has obviously inhibition to osteoclast formation and has a dose-dependence manner. |
| KEYWORDS:Osteoclast Simvastatin Cell culture |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 黄鲁豫,胡蕴玉,马真胜,吕荣.辛伐他汀抑制甲状旁腺素相关肽诱导的破骨细胞生成和功能[J].中国骨伤,2006,19(7):420~423 |
| 英文格式: | HUANG Luyu,HU Yun-yu,LV Rong,HUANG Luyu.Inhibition of simvastatin on osteoclast formation and function induced by PTHrP[J].zhongguo gu shang / China J Orthop Trauma ,2006,19(7):420~423 |
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