| 大鼠骶髂关节传入神经通路的实验研究 |
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Received:June 14, 2005
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| 作者 | Author | 单位 | Unit | E-Mail |
| 洪盾 |
HONG Dun |
台州医院脊柱外科,浙江台州317000 |
Department of Spinal Surgery,Taizhou Hospital,Taizhou 317000,Zhejiang,China |
LHHQ@yahoo.com.cn |
| 顾海锋 |
GU Hai-feng |
浙江大学附属邵逸夫医院骨科 |
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| 方向前 |
FANG Xiang-qian |
浙江大学附属邵逸夫医院骨科 |
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| 陈海啸 |
CHEN Hai-xiao |
台州医院脊柱外科,浙江台州317000 |
Department of Spinal Surgery,Taizhou Hospital,Taizhou 317000,Zhejiang,China |
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| 范顺武 |
FAN Shun-wu |
浙江大学附属邵逸夫医院骨科 |
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| 期刊信息：《中国骨伤》2006年19卷，第1期，第25-27页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
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目的:通过逆行神经追踪法研究大鼠骶髂关节的传入神经通路。
方法:30只雄性Sprague-Dawley大鼠随机分成非交感神经切除组(A组)和交感神经切除组(B组),每组15只,交感神经切除组切除左侧L1以下椎旁交感干。两组左侧骶髂关节注入30%的辣根过氧化物酶(HRP)20μl,72h后取出双侧的L1-S1背根神经节(DRG),TMB法染色后在光学显微镜下观察HRP阳性神经元细胞并计数。
结果:两组左侧L1、L2背根神经节内HRP阳性神经元差异有显著性意义(P<0.05),B组HRP阳性神经元明显减少;左侧L3-S1背根神经节内HRP阳性神经元差异无显著性意义(P>0.05)。
结论:L1-S1神经节含有支配同侧骶髂关节的传入神经元,同侧椎旁交感干可能是骶髂关节到L1-L2神经节重要的传入神经旁路,而不是到L3-L5神经节的传入旁路或重要的传入旁路。 |
| [关键词]:骶髂关节 神经支配 背根神经节 辣根过氧化物酶 |
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| Experimental study on afferent pathways of rat sacroiliac joint |
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Abstract:
Objective: To investigate afferent pathways of rat sacroiliac joint with neural retro-tracing technique.
Methods:Thirty male Sprague-Dawley rats were randomly divided into sympathectomy group（group A,of which left paravertebral sympathetic trunk were cut down）and unsympathectomy group（group B）.20 μl 30% horseradish peroxidase （HRP） was injected into left sacroiliac joints in two groups.After 72 hours ,bilateral L1-S1 dorsal root ganglia （DRG） were harvested and processed by TMB method, in which HRP??positiv e neurons were observed and counted under lig ht microscope.
Results:There were significant differences in the number of HRP??positive neurons in left L1, L2 DRG between group A and group B ( P<0.05). HRP??positive neurons in group B obviously decrease than g roup A. However , there was no significant differ ences in left L3-S1 DRG w er e observed betw een group A and group B (P> 0.05).
Conclusion:Afferent neurons of govern same side sacroiliac joint were included by L1-S1 DRG. The same side paravetebral sympathetic trunk might be an impor tant neural pathway between sacroiliac joint and L1, L2 DRG. How ever, it might be not a key bypass from sacroiliac joint to L3-S1 DRG. |
| KEYWORDS:Sacroiliac joint Innervation Dorsal root ganglion Horseradish peroxidase |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 洪盾,顾海锋,方向前,陈海啸,范顺武.大鼠骶髂关节传入神经通路的实验研究[J].中国骨伤,2006,19(1):25~27 |
| 英文格式: | HONG Dun,GU Hai-feng,FANG Xiang-qian,CHEN Hai-xiao,FAN Shun-wu.Experimental study on afferent pathways of rat sacroiliac joint[J].zhongguo gu shang / China J Orthop Trauma ,2006,19(1):25~27 |
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