| 应用血管内皮生长因子基因治疗股骨头缺血性坏死的实验研究 |
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Received:March 16, 2005
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| 期刊信息：《中国骨伤》2005年18卷，第10期，第604-606页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:国家自学科学基金项目(编号:30170945) |
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目的:通过建立犬股骨头缺血坏死模型,探讨应用脱蛋白骨复合转染血管内皮生长因子(vascular endothelial growthfactor,VEGF)基因骨髓基质细胞植入治疗股骨头缺血坏死的可行性。
方法:实验选取36只成年犬,随机分为3组,每组12只。A组为脱蛋白异体骨复合转染VEGF基因的自体骨髓基质细胞;B组为脱蛋白异体骨复合未转染基因的自体骨髓基质细胞;C组单纯植入脱蛋白骨材料。采用液氮冷冻法制作狗股骨头缺血坏死模型,将细胞骨材料复合物植入缺血坏死股骨头内。应用微循环灌注方法了解股骨头内的血运情况,组织形态学观察坏死股骨头的修复情况,免疫组化方法检测VEGF的表达,骨密度仪测定股骨头的骨密度。
结果:A组4周后股骨头内有VEGF表达,12周后股骨头内有大量的树枝状血管生成,大量新骨形成,骨密度增高;B、C组均无VEGF基因表达,B组有部分新骨及血管生成,C组仅见少量类骨质形成,血管再生不明显。
结论:利用脱蛋白骨复合转染VEGF基因的骨髓基质细胞可促进新骨形成与血管再生,有利于促进坏死骨的修复。 |
| [关键词]:血管内皮生长因子 基因疗法 组织工程 股骨头缺血性坏死 动物,实验 |
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| Treatment of avascular necrosis of the femoral head with VEGF165 gene |
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Abstract:
Objective:To explore possibility of a new therapy for avascular necrosis of the femoral head using deproteinized bone （DPB） delivery of marrow stromal cells （MSCs）transfected with VEGF 165 gene.
Methods:36 adult dogs were evenly randomized into three groups according to the materials for implantation.Group A was implanted with DPB delivery of MSCs transfected with VEGF165 gene;group B was implanted with DPB delivery of MSCs without transfection of VEGF165 gene;group C was implanted with DPB only.The avascular necrosis model was made on left femoral head by use of liquefied nitrogen.The expression of VEGF165 gene was examined by immunohistochemical method.Angiogenesis of the femoral head was detected by the method of microcirculatory perfusion.Repairing of the femoral head was observed by histological method and bone density of the femoral head was determined by bone-density-measuring machine.
Results:The expression of VEGF165 gene was detectable in the group A,but not in the other two groups,4 weeks after the operation.Three branch-shaped angiogenesis was more abundant and bone density was higher in the group A than those in the other two groups 12 weeks after the operation.Bone repairing was also much quicker in the group A as compared with the other two groups.
Conclusion:DPB delivery of MSCs transfected with VEGF165 gene cantonly augment angiogenesis and improve microcirculation,but also accelerate bone repairing.This therapy is hopeful for the treatment of avacular osteonecrosis. |
| KEYWORDS:Vascular endothelial growth factor Gene therapy Tissue engineering Avascular necrosis of femur head Animals,laboratory |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 刘日光,杨述华,易诚青,刘建湘,杨操.应用血管内皮生长因子基因治疗股骨头缺血性坏死的实验研究[J].中国骨伤,2005,18(10):604~606 |
| 英文格式: | LIU Ri-guang,YANG Shu-hua,YI Cheng-qing,LIU Jian-xiang,YANG Cao.Treatment of avascular necrosis of the femoral head with VEGF165 gene[J].zhongguo gu shang / China J Orthop Trauma ,2005,18(10):604~606 |
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