| 骨宝液在去卵巢大鼠骨质疏松症中抑制骨转换作用的实验研究 |
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Received:April 25, 2005
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| 作者 | Author | 单位 | Unit | E-Mail |
| 张兆华 |
ZHANG Zhao-hua |
广州中医药大学附属佛山市中医院骨科,广东佛山528000 |
Department of Orthopaedic Surgery,Foshan Hospital of TCM,Foshan Guangdong,528000,China |
MDCD@fstcm.com.cn |
| 陈志维 |
CHEN Zhi-wei |
广州中医药大学附属佛山市中医院骨科,广东佛山528000 |
Department of Orthopaedic Surgery,Foshan Hospital of TCM,Foshan Guangdong,528000,China |
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| 陈逊文 |
CHEN Xun-wen |
广州中医药大学附属佛山市中医院骨科,广东佛山528000 |
Department of Orthopaedic Surgery,Foshan Hospital of TCM,Foshan Guangdong,528000,China |
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| 罗汉文 |
LUO Han-wen |
广州中医药大学附属佛山市中医院骨科,广东佛山528000 |
Department of Orthopaedic Surgery,Foshan Hospital of TCM,Foshan Guangdong,528000,China |
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| 期刊信息：《中国骨伤》2005年18卷，第8期，第473-476页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:广东省中医药管理局科研课题(编号:1040177) |
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目的:探讨骨宝液对去卵巢大鼠骨质疏松症中抑制骨转换作用。
方法:60只大鼠去卵巢诱导骨质疏松症,随机分为正常对照组、模型组、尼尔雌醇组、骨宝液低、中、高剂量组。正常对照组、模型组以0.9%生理盐水每天10ml/kg,尼尔雌醇组以尼尔雌醇每2周1mg/kg,其他三组每天分别以骨宝液5、101、5ml/kg的剂量灌胃给药3个月后,以比色法测定其血清钙(s-Ca)、骨碱性磷酸酶(b-AKP)和血清抗酒石酸酸性磷酸酶(s-TRAP)的活性,竞争放射免疫法测定血清中骨钙素(s-BGP)的含量;双能X线骨密度仪(DEXA)测定股骨及腰椎的骨密度;三点弯曲试验法测定骨生物力学;扫描电镜进行骨小梁形态计量学分析。
结果:骨宝液组与其他组比较,s-TRAP水平降低,腰椎骨密度增加,股骨抗弯抗拉能力改善,骨小梁形态改善,骨小梁平均宽度(MTT)与骨小梁面积百分比(TS%)显著增加。
结论:骨宝液对去卵巢大鼠骨质疏松症具有防治作用,其机制可能与抑制骨转换有关。 |
| [关键词]:卵巢切除术 骨质疏松 中药 |
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| The experimental study of the effects of Gubaoye(骨宝液)on osteoporosis in ovariectomized rats by inhibiting the bone turnover |
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Abstract:
Objective:To explore the effects of Gubaoye on osteoporosis in ovariectomized rats by inhibiting the bone turnover.
Methods:Sixty rats were induced to osteoporosis by ovariectomy,and randomly divided into six groups(ten rats in each group):A control group,a model group,a Nilestriol group,a low-dose-Gubaoye group,a middle-Gubaoye group and a high-dose-Gubaoye group.The control and model groups were fed with 0.9% saline 10 ml/kg once a day.The Nlestriol group was fed with 1 mg/kg.The other three groups were fed with Gubaoye at the dose of 5,10,15 ml/kg once a day respectively.After fedding for 3 months,the concentration of calcium (s-Ca),tartrate resistant acid phosphatase in serum (s-TRAP)and alkaline phosphatase in bone (b-AKP)were measured by colorimetric method.The concentration of osteocalcin in serum (s-BGP)was measured by competitive radio immunologic method.Bone mineral density (BMD) of femur and lumbar of rats was determined by Dual Engergy X-ray Absorbtiometry (DEXA).Biomechanical characteristics of femur were measured by three point bending test.Histomorphometry of bone trabecula was analysed by scanning electron microscope.
Results:As compared with the other groups,In the ubaoye groups,the level of s-TRAP was decreased;The bone density of lumbar vertebra was increased;The resistance of bending and compression of fumr of rats was enhanced;The form of bone trabecula was impoved and MTT and trabeculae square percentage (TS%) were obviously increased,as compared with other groups.
Conclusion:Gubaoye has anti-osteoporotic effects on ovariectomized rats by inhibiting the bone turnover. |
| KEYWORDS:Ovariectomy Osteoporosis Chinese herbal |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 张兆华,陈志维,陈逊文,罗汉文.骨宝液在去卵巢大鼠骨质疏松症中抑制骨转换作用的实验研究[J].中国骨伤,2005,18(8):473~476 |
| 英文格式: | ZHANG Zhao-hua,CHEN Zhi-wei,CHEN Xun-wen,LUO Han-wen.The experimental study of the effects of Gubaoye(骨宝液)on osteoporosis in ovariectomized rats by inhibiting the bone turnover[J].zhongguo gu shang / China J Orthop Trauma ,2005,18(8):473~476 |
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