| 马钱子对三种软骨细胞凋亡模型的影响 |
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Received:November 02, 2004
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| 作者 | Author | 单位 | Unit | E-Mail |
| 张梅 |
ZHANG Mei |
安徽省立医院,安徽合肥230001 |
Anhui Prinvince Hospital,Anhui Hefei,230001,China |
zhang69@sohu.com |
| 李平 |
LI Ping |
安徽省立医院,安徽合肥230001 |
Anhui Prinvince Hospital,Anhui Hefei,230001,China |
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| 汪健 |
WANG Jian |
安徽省立医院,安徽合肥230001 |
Anhui Prinvince Hospital,Anhui Hefei,230001,China |
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| 翟志敏 |
ZHAI Zhi-min |
安徽省立医院,安徽合肥230001 |
Anhui Prinvince Hospital,Anhui Hefei,230001,China |
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| 陈朝晖 |
CHEN Zhao-hui |
安徽中医学院 |
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| 期刊信息：《中国骨伤》2005年18卷，第7期，第410-412页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:国家中医药管理局课题(编号:2000-JP-58) |
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目的:观察中药马钱子对三种软骨细胞凋亡模型的影响。
方法:在兔软骨细胞培养体系中分别加入2mmol/L硝普钠(SNP)、2mmol/LSNP+马钱子、10-4mol/L全反式维甲酸(ATRA)、10-4mol/LATRA+马钱子;在人胚胎软骨细胞培养体系中加入30ng/L肿瘤坏死因子α(TNFα)、TNFα+马钱子,各组培养24h后,采用AnnexinV/PI双参数法对软骨细胞凋亡进行定量检测。
结果:软骨细胞在加入SNP、ATRA、TNFα发生凋亡时,加入马钱子能降低SNP、ATRA诱导的软骨细胞凋亡率(P<0.01,P<0.05),但对TNFα诱导的软骨细胞凋亡,无明显抑制作用(P>0.05)。
结论:中药马钱子对部分软骨细胞凋亡有一定抑制作用。 |
| [关键词]:马钱子 软骨细胞 凋亡 |
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| Effects of Semen strychni on three types of chondrocytes apoptosis |
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Abstract:
Objective:To evalute the effects of Semen strychni on three types of chondrocytes apoptosis.
Methods:Chondrocytes of rabbits were respectively cultured with ①2 mmol/L sodium nitroprusside（SNP）,②Semen strychni and 2 mmol/L SNP,③10-4 mol/L all-trans retinoic acid （ATRA）,④Semen strychni and 10-4 mol/L ATRA.Fetac chondrocytes were respectively cultured with ①30 ng/L tumor necrosis factor-α（TNF-α）,②Semen strychni and 30 ng/L TNF-α.The rates of chondrocytes apoptosis in every group were detected by Annexin V/PI with flow cytometry after being cultured for 24 h.
Results:The chondrocyte apoptosis could be induced by SNP,ATRA and TNF-α respectively.Adding Semen strychni into cultured could decrease chondrocytes apoptosis induced by SNP?ATRA（P<0.01,P<0.05）,but had no effects on apoptosis induced by TNF-α（P>0.05）.
Conclusion:Semen strychni has inhibitory effects on some chondrocyte apoptosis. |
| KEYWORDS:Semen strychni Chondrocyte Apoptosis |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 张梅,李平,汪健,翟志敏,陈朝晖.马钱子对三种软骨细胞凋亡模型的影响[J].中国骨伤,2005,18(7):410~412 |
| 英文格式: | ZHANG Mei,LI Ping,WANG Jian,ZHAI Zhi-min,CHEN Zhao-hui.Effects of Semen strychni on three types of chondrocytes apoptosis[J].zhongguo gu shang / China J Orthop Trauma ,2005,18(7):410~412 |
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