| 参麦液对早期退变腰椎间盘组织中NO、TGF-β1表达的影响 |
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Received:June 08, 2004
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| 作者 | Author | 单位 | Unit | E-Mail |
| 林定坤 |
LIN Ding-kun |
广东省中医院骨科 广东广州510120 |
Department of Orthopaedics,TCM Hospital of Guangdong Province,Guangdong Guangzhou,510120,China |
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| 郭玉海 |
GUO Yu-hai |
广东省中医院骨科 广东广州510120 |
Department of Orthopaedics,TCM Hospital of Guangdong Province,Guangdong Guangzhou,510120,China |
drguoyuhai@sohu.com |
| 陈博来 |
CHEN Bo-lai |
广东省中医院骨科 广东广州510120 |
Department of Orthopaedics,TCM Hospital of Guangdong Province,Guangdong Guangzhou,510120,China |
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| 孔畅 |
KONG Chang |
广东省中医院骨科 广东广州510120 |
Department of Orthopaedics,TCM Hospital of Guangdong Province,Guangdong Guangzhou,510120,China |
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| 杨仁轩 |
YANG Ren-xuan |
广东省中医院骨科 广东广州510120 |
Department of Orthopaedics,TCM Hospital of Guangdong Province,Guangdong Guangzhou,510120,China |
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| 赵帅 |
ZHAO Shuai |
广东省中医院骨科 广东广州510120 |
Department of Orthopaedics,TCM Hospital of Guangdong Province,Guangdong Guangzhou,510120,China |
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| 黄刚 |
HUANG Gang |
广东省中医院骨科 广东广州510120 |
Department of Orthopaedics,TCM Hospital of Guangdong Province,Guangdong Guangzhou,510120,China |
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| 期刊信息：《中国骨伤》2005年18卷，第3期，第142-143页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
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目的:探讨参麦液在延缓椎间盘退变中的作用。
方法:对6例年轻腰椎间盘突出患者的椎间盘组织采取体外细胞培养,随机分为4组:对照组、1%参麦液组、2.5%参麦液组、5%参麦液组,用分光光度法测定各组培养上清液中NO含量与NOS活性,并采用免疫组化的研究方法测定椎间盘细胞TGFβ1表达。
结果:对照组退变椎间盘组织产生的NO为(14.41±9.7)μmol/L,NOS为(6.2±0.6)U/ml,高于参麦液组,有统计学差异。参麦液组中的椎间盘细胞呈不同程度的棕黄色,对照组则呈阴性改变。
结论:参麦液可降低椎间盘内炎症因子的浓度,促进椎间盘细胞TGF-β1表达,有效减缓椎间盘组织的退变。 |
| [关键词]:一氧化氮 一氧化氮合酶 转化生长因子-β1 细胞培养 中成药 |
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| Effect of Shenmai decoctionon the expression of NO and TGF-β1 of degenerative lumbar intervertebral disc tissue in earlier period |
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Abstract:
Objective:To investigate the action of Shenmai liquid in defering the degeneration of the human intervertebral discs.
Methods:Six herniated lumbar discs of the youths were placed into cell culture and randomly distributed into four groups:the control group,the 1,2.5 and 5 percent Shenmai decoction groups.The incubation supernatants were used to detect the nitric oxide(NO)and the nitric oxide synthase(NOS)concentration with the method of spectrophotography,and the transforming growth factor-β 1(TGF-β 1)expression of the intervertebral disc cells were estimated with immunohistochemical method.
Results:The contents of NO and NOS in the control group were marked higher than those in the Shenmai decoction groups.The intervertebral disc cells in the Shenmai decoction groups showed obviously brown,and the control were negative.
Conclusion:Shenmai decoction can decrease the concentration of the inflammatory factors and promote the expression of TGF-β 1 of the human intervertebral discs,which can defer the degenetation of the human intervertebral discs. |
| KEYWORDS:Nitric oxide Nitric-oxide synthase Transforming growth factor-β 1 Cell culture Chinese patent drugs |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 林定坤,郭玉海,陈博来,孔畅,杨仁轩,赵帅,黄刚.参麦液对早期退变腰椎间盘组织中NO、TGF-β1表达的影响[J].中国骨伤,2005,18(3):142~143 |
| 英文格式: | LIN Ding-kun,GUO Yu-hai,CHEN Bo-lai,KONG Chang,YANG Ren-xuan,ZHAO Shuai,HUANG Gang.Effect of Shenmai decoctionon the expression of NO and TGF-β1 of degenerative lumbar intervertebral disc tissue in earlier period[J].zhongguo gu shang / China J Orthop Trauma ,2005,18(3):142~143 |
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