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黏附因子N-cadherin在体外骨髓间质细胞诱导分化中的表达

Hits: 1848 Download times: 1821 Received:July 19, 2004

作者	Author	单位	Unit	E-Mail
李文革	LI Wen-ge	北京大学深圳医院骨科,广东深圳518036	Peking Uinversity ShenZhen Hospital
李继云	LI Ji-yun	北京大学深圳医院骨科,广东深圳518036	Peking Uinversity ShenZhen Hospital
汤晨逢	TANG Chen-feng	北京大学深圳医院骨科,广东深圳518036	Peking Uinversity ShenZhen Hospital
杨昀焯	YANG Yun-zhuo	北京大学深圳医院骨科,广东深圳518036	Peking Uinversity ShenZhen Hospital
徐莘香	XU Shen-xiang	吉林大学第一临床医院骨科

期刊信息：《中国骨伤》2004年17卷，第11期，第656-658页

DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz

目的:探讨黏附因子Ncadherin在骨髓间质细胞诱导分化中的表达及其转化机制。

方法:体外分离,扩增Wistar大鼠骨髓间质细胞,按一定浓度种植于24孔板内,并加入TGFβ诱导剂,于不同时间点取材固定。用免疫组化方法动态观察Ncadherin在骨髓间质细胞诱导分化中表达时相。同时加入抗Ncadherin抗体干扰细胞聚集。

结果:Ncadherin主要表达于加入诱导剂后24h,之后呈下调控趋势,并呈现时间依赖性。加入抗体后可明显干扰细胞聚集,当浓度为300μl/孔时,未见细胞小结形成。

结论:Ncadherin介导了细胞与细胞之间的相互作用,在体外间质细胞转化中发挥重要作用。

[关键词]:间质干细胞钙黏蛋白凝集软骨发生

Expression of N-cadherin in mesenchymal stem cells by inducing differentiation of TGF-β

Abstract:

Objective:To investigate the expression of N-cadherin by inducing differentiation of TGF-β 1.

Methods:The mesenchymal stem cells of Wistar Rats were harvested in vitro and implanted in 24-hole plates in a monolayer culture as well as fixed at different timepoint after adding inducer TGF-β₁,immunohistochemistry was applied to observe the time-phase of expression of N-cadherin during cell transformation,and with its anti-body to disturb aggregation of cells.Result:N-cadherin was expressed only in 24h after adding TGF-β₁,and then down-regulation and time-dependant phase were be showed,its antibody could inbibit the cellular aggregation completely.The optimal dose was 300 ul/hole.

Conclusion:N-cadherin mediates interaction among cells,it is an important role playing in the differentiation of cells.

KEYWORDS:Mesenchymal stem cells N-cadherin Condensation Chondrogenesis

引用本文，请按以下格式著录参考文献：

中文格式:	李文革,李继云,汤晨逢,杨昀焯,徐莘香.黏附因子N-cadherin在体外骨髓间质细胞诱导分化中的表达[J].中国骨伤,2004,17(11):656~658
英文格式:	LI Wen-ge,LI Ji-yun,TANG Chen-feng,YANG Yun-zhuo,XU Shen-xiang.Expression of N-cadherin in mesenchymal stem cells by inducing differentiation of TGF-β[J].zhongguo gu shang / China J Orthop Trauma ,2004,17(11):656~658

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