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中药黄芪对脊髓星形胶质细胞单核细胞趋化蛋白-1分泌的影响

Hits: 2414 Download times: 1567 Received:January 13, 2004

作者	Author	单位	Unit	E-Mail
马永刚	MA Yong-gang	武汉大学人民医院骨科,湖北武汉430060	Department of Orthopaedics,Renmin Hospital of Wuhan University	Martin 1354@163.com
刘世清	LIU Shi-qing	武汉大学人民医院骨科,湖北武汉430060	Department of Orthopaedics,Renmin Hospital of Wuhan University
刘敏	LIU Min	武汉大学医学院免疫学教研室
彭昊	PENG Hao	武汉大学人民医院骨科,湖北武汉430060	Department of Orthopaedics,Renmin Hospital of Wuhan University

期刊信息：《中国骨伤》2004年17卷，第11期，第653-655页

DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz

目的:研究黄芪对体外培养的脊髓星形胶质细胞趋化蛋白1(MCP-1)分泌的影响。

方法:自Wistar大鼠脊髓组织分离、纯化星形胶质细胞,体外培养,分别予以TNF-α及TNFα+黄芪处理,ELISA方法检测培养上清液中MCP-1的表达。

结果:TNF-α可以显著刺激星形胶质细胞合成、释放MCP-1,而黄芪可下调其刺激作用。

结论:受炎症因子刺激活化的星形胶质细胞可能是损伤脊髓局部MCP-1的来源之一,黄芪可以减少脊髓损伤后内源性MCP-1的产生,从而缓解继发性脊髓损伤,发挥脊髓保护作用。

[关键词]:黄芪单核细胞趋化蛋白-1 脊髓损伤星形胶质细胞

Effects of astragalus root on the secretion of MCP-1 by astrocytes in vitro from spinal cord of adult rats

Abstract:

Objective:To study the secretion of MCP-1 by astrocytes culturted in vitro and the possible influence of astragalus root(AR).

Methods:Astrocytes were separated and purified from spinal cord of adult Wistar rats.The expression of MCP-1 was detected by ELISA when the astrocyte were culturted in vitro with TNF-α or combined with AR.

Results:TNF-α markedly increase the secretion of astrocyte-derived MCP-1 which could be down-regulated by AR to some extent.

Conclusion:Astrocytes activated by some inflammatory factors may be one of the sources of MCP-1 secreted in the local injuried spinal cord.AR probably can decrease the engenous expression of MCP-1 and thus show a neuroprotective effects.

KEYWORDS:Astragalus root Monocyte chemoattractant protein-1(MCP-1) Spinal injuries Astrocyte

引用本文，请按以下格式著录参考文献：

中文格式:	马永刚,刘世清,刘敏,彭昊.中药黄芪对脊髓星形胶质细胞单核细胞趋化蛋白-1分泌的影响[J].中国骨伤,2004,17(11):653~655
英文格式:	MA Yong-gang,LIU Shi-qing,LIU Min,PENG Hao.Effects of astragalus root on the secretion of MCP-1 by astrocytes in vitro from spinal cord of adult rats[J].zhongguo gu shang / China J Orthop Trauma ,2004,17(11):653~655

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