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二磷酸盐对关节磨屑刺激单核细胞分泌IL-1β的抑制作用

Hits: 1973 Download times: 1145 Received:December 30, 2003

作者	Author	单位	Unit	E-Mail
陈明	CHEN Ming	华中科技大学同济医学院附属同济医院骨科, 湖北武汉 430030	Department of Orthopaedics, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology	cm19720@163.com
夏仁云	XIA Ren-yun	华中科技大学同济医学院附属同济医院骨科, 湖北武汉 430030	Department of Orthopaedics, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology

期刊信息：《中国骨伤》2004年17卷，第10期，第596-598页

DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz

目的:研究二磷酸盐对关节磨屑刺激单核细胞分泌IL-β的影响。

方法:分离培养人外周血单核细胞,加入关节磨屑及不同浓度的阿伦膦酸钠,ELISA检测细胞上清中IL-1β的含量,原位杂交检测IL-1βmRNA的表达。

结果:关节磨屑组IL-1β及基因的表达显著高于对照组(P<0.01),不同浓度的阿伦膦酸钠组IL-1β及基因的表达明显低于关节磨屑组(P<0.01)。

结论:阿伦膦酸钠能通过下调人外周血单核细胞IL-1β3及mRNA的表达,对人工关节松动可能起防治作用。

[关键词]:二磷酸盐关节磨屑白细胞介素-1

Inhibitory effect of bisphosphonates on IL-1β expression of human monocytes induced by wear debris

Abstract:

Objective:To study the effect of bisphosphonates on IL-1β expression of human monocytes with wear particles stimulation.

Methods: In vitro human peripheral blood mononuclear cell (PBMC) were cultured with wear particles and bisphosphonates of different concentration, then productions of IL-1β and IL-1β mRNA were determined by ELISA and in situ hybridization techniques.

Results:The production of IL-1β were increased significantly when wear particles was added,compared with control group (P<0.01). And bisphosphonates reduced the level of IL-1β. Hybridization demonstrated bisphosphonates might down regulate IL-1β mRNA expression.

Conclusion:Bisphosphonates can inhibit IL-1β expression induced by wear debris and may be used in the prevention and treatment of aseptic loosening of prosthesis.

KEYWORDS:Bisphasphonates Wear debris Interleukin-1

引用本文，请按以下格式著录参考文献：

中文格式:	陈明,夏仁云.二磷酸盐对关节磨屑刺激单核细胞分泌IL-1β的抑制作用[J].中国骨伤,2004,17(10):596~598
英文格式:	CHEN Ming,XIA Ren-yun.Inhibitory effect of bisphosphonates on IL-1β expression of human monocytes induced by wear debris[J].zhongguo gu shang / China J Orthop Trauma ,2004,17(10):596~598

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