| 中药增骨丸对去卵巢大鼠骨髓IGF-1和IL-1β的影响 |
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Received:March 09, 2004
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| 期刊信息：《中国骨伤》2004年17卷，第9期，第524-526页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:甘肃省自然科学基金资助项目(ZS011A25059Y) |
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目的:探讨去卵巢后大鼠骨髓上清中胰岛素样生长因子-1(IGF-1)和白细胞介素-1β(IL-1β)的变化及中药增骨丸对其影响。
方法:将48只8个月龄Wistar雌性大鼠随机分为正常对照组、模型组、乙烯雌酚组及3种不同浓度的增骨丸组。每组8只。正常组仅摘除一段肠系膜,其余5组均做双侧卵巢切除术。术后1周开始给药,连续用药90d后取右侧股骨,剔净软组织,截取15cm,用1mlPBS液将骨髓冲入DEPC处理过的试管中备用。分别测定骨髓中IGF-1和IL-1β的含量。
结果:卵巢切除后大鼠骨髓上清中IGF-1的含量明显降低,而IL-1β的含量明显增高。经过雌激素及中药治疗能明显增高卵巢切除所致的IGF1的降低,抑制IL-1β的增高,且其作用与剂量有关系。
结论:增骨丸通过增加IGF1的表达和降低IL1β的表达而发挥防治骨质疏松症的作用。 |
| [关键词]:骨质疏松 胰岛素样生长因子I 白细胞介素-1β 中成药 |
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| Effect of Zenggu pill on IGF-1 and IL-1β in bone medullae of ovariectomized rats |
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Abstract:
Objective:To inquire into effects of Zenggu pill (ZGP) on bone medullae insulin-like growth factor-1 and Interleukin-1β in ovariectomized rats.
Methods:Forty-eight healthy female Wistar rats were randomly divided into six groups:normal control group,model group,positive group and three ZGP groups (with high,moderate and low dosages respectively).The model group was excised a small part of mesentery and the other groups were ovariectomized bilaterally.They were put to death at the same time after three months.Then took right femur,measured the content of insulin-like growth factor-1 and Interleukin-1β in bone medullae by RIA and ELISA.
Results:The content of insulin-like growth factor-1 in bone medullae of the high,moderate dosage of ZGP and positive control group rats were more higher than those in the model group (P<0.05),but the content of Interleukin-1β were much lower than that in the model group.
Conclusion:The high and moderate ZGP can increase the content of insulin-like growth factor-1 and decrease Interleukin-1β in bone medullae.So they have the effect of prevention and treatment to postmenopausal osteoporosis,and the effects might be related to the level of doses of ZGP. |
| KEYWORDS:Osteoporosis Insulin-like growth factor I Interleukin-1β Chinese patent drugs |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 李宁,谢兴文,宋敏,张晓刚,宋鹏程.中药增骨丸对去卵巢大鼠骨髓IGF-1和IL-1β的影响[J].中国骨伤,2004,17(9):524~526 |
| 英文格式: | LI Ning,XIE Xing-wen,SONG Min,ZHANG Xiao-gang,SONG Peng-cheng.Effect of Zenggu pill on IGF-1 and IL-1β in bone medullae of ovariectomized rats[J].zhongguo gu shang / China J Orthop Trauma ,2004,17(9):524~526 |
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