| 实验性无创兔颈椎间盘退变模型的建立 |
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Received:January 09, 2004
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| 期刊信息：《中国骨伤》2004年17卷，第8期，第466-470页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:浙江省教育厅资助项目(No.20010018);浙江省中医药管理局资助项目(No.2002-46) |
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目的:观察长时间异常应力环境下兔颈椎间盘的组织形态、基质生化成分和生物力学性能变化,为防治颈椎病的研究提供动物模型和实验依据。
方法:选取35只家兔,随机分为对照组(20只)、模型组(15只),造模家兔颈椎处于低头屈曲45°位异常应力环境下5h/d。动物处死后:①颈椎间盘光镜和电镜下观察组织形态学变化;②免疫组化和生化方法测定基质胶原和蛋白聚糖(proteoglycan,PG)含量和成分的变化;③功能节段生物力学性能测试。比较各组间可能存在的差异。
结果:①对照组颈椎间盘组织形态、基质胶原和PG含量和成分及生物力学性能无明显变化;②模型组颈椎间盘发生软骨细胞变性、坏死,髓核皱缩,纤维环胶原纤维变性、排列紊乱,软骨终板钙化、断裂,胶原纤维排列紊乱等组织形态学改变。髓核和软骨终板中PG总量、硫酸软骨素(chondroitinsulphate,CS)含量和Ⅱ型胶原表达下降,纤维环中Ⅰ型胶原表达增加,椎间盘压缩、扭转生物力学性能下降,并随着异常应力作用时间的延长而表现更为显著。
结论:长时间处于异常应力环境下能使兔颈椎间盘组织形态、基质生化成分及生物力学性能均发生明显退行性改变,成功建立家兔颈椎间盘退变模型。 |
| [关键词]:椎间盘 退变 模型,动物 应力,物理 |
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| Establishment of rabbits experimental model for cervical intervertebral disc degeneration |
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Abstract:
Objective:To establish a new cervical intervertebral disc degeneration animal model by characterizing the changes of cervical intervretebral disc histopathology,biochemistry and biomechanics associated with disc degeneration through keeping the rabbits cervical spine into forty-five-degree flexion for five hours once a day.
Methods:Thirty-five Japanese white rabbits were randomly divided into control and model groups.Rabbits of the model group were kept at forty-five-degree flexion for five hours once a day.After sacrificed:① Observing the histopathological changes under light microscopy and transmission electronic microscopy;② Determining collagen type Ⅰ,Ⅱ and proteoglycan(PG)components content;③ The regular biomechanical test made on the function of spinal unit.
Results:① Rabbits in the control group showed no histopathological,biochemical and biomechanical changes on cervical intervertebral disc;② In the model group,the pathological changes of the intervertebral disc including:Spit of the annulus fibrosis,dehydration of pulposus nucleus,derangement of the collagen of the annulus fibrosis,calcification,ossification and separation of the cartilage endplate;PG content in the pulposus nucleus and the cartilage endplate decreased significantly,while collagen type Ⅰ expression increased.Biomechanical test showed an significant decrease of resist-compression character of the intervertebral disc.The longer the flexion time,the more significant the results were.
Conclusion:A new in vivo animal model was established by keeping the cervical spine into the abnormal stress condition. |
| KEYWORDS:Intervertebral disc Degeneration Models,animal Stress,mechanical |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 应航,陈立,詹红生,井夫杰,吕荣坤.实验性无创兔颈椎间盘退变模型的建立[J].中国骨伤,2004,17(8):466~470 |
| 英文格式: | YING Hang,CHEN Li,ZHAN Hong-sheng,JING Fu-jie,Lü Rong-kun.Establishment of rabbits experimental model for cervical intervertebral disc degeneration[J].zhongguo gu shang / China J Orthop Trauma ,2004,17(8):466~470 |
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