| 活血补肾中药对培养成骨细胞VEGF活性的影响 |
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Received:April 21, 2003
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| 期刊信息：《中国骨伤》2004年17卷，第5期，第260-262页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:贵州省自然科学基金资助项目[计字(2001)3049] |
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目的:研究活血补肾中药丹仙康骨胶囊对体外培养成骨细胞VEGF活性的影响,进一步探讨丹仙康骨胶囊抗骨坏死的作用机制。
方法:应用RT-PCR及流式细胞仪检测观察不同剂量丹仙康骨胶囊对成骨细胞VEGFmRNA的表达与VEGF的生成的影响。
结果:丹仙康骨胶囊可以促进成骨细胞VEGFmRNA的表达与VEGF的生成,并呈剂量依赖性。
结论:丹仙康骨胶囊促进成骨细胞VEGFmRNA的表达与VEGF的生成可能是其抗骨坏死的作用机制之一。 |
| [关键词]:丹仙康骨胶囊 成骨细胞 血管内皮生长因子 |
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| Effects of Danxian Kanggu capsule on the vascular endothelial growth factor expression and production in osteoblasts |
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Abstract:
Objective:To investigate the effects of Danxian Kanggu capsules extracted from traditional Chinese medicine on the vascular endothelial growth factor expression and production by osteoblats,and explore the mechanism of anti-osteonecrosis of Danxian Kanggu capsules.
Methods:Osteoblasts derived from fetal rats were used in this study.VEGF mRNA expression,intracellular production induced by Danxian Kanggu capsules were measured by RT-PCR and flow cytometry.
Results:Danxian Kanggu capsules could significantly increase vascular endothelial growth factor expression and production in osteoblats in a dose-dependent manner.
Conclusion:The anti-osteonecrosis effect of Danxian Kanggu capsules may be through its increasable effect on vascular endothelial growth factor expression and production in osteoblasts. |
| KEYWORDS:Danxian Kanggu capsule Osteoblast Endothelial growth factor |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 沈冯君,刘日光,杨述华,沈骏,易诚青,李新春,刘建湘.活血补肾中药对培养成骨细胞VEGF活性的影响[J].中国骨伤,2004,17(5):260~262 |
| 英文格式: | SHEN Feng-jun,LIU Ri-guang,YANG Shu-hua,SHEN Jun,YI Cheng-qin,LI Xin-chun,LIU Jian-xiang.Effects of Danxian Kanggu capsule on the vascular endothelial growth factor expression and production in osteoblasts[J].zhongguo gu shang / China J Orthop Trauma ,2004,17(5):260~262 |
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