| 葛根汤和桂枝汤调节椎间盘组织Fas、bcl-2蛋白表达的实验研究 |
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Received:September 17, 2003
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| 作者 | Author | 单位 | Unit | E-Mail |
| 刘梅 |
LIU Mei |
上海中医药大学脊柱病研究所,上海200032 |
Institute of Spondylopathy,Shanghai University of TCM |
LiuMei926@sina com |
| 王拥军 |
WANG Yong-jun |
上海中医药大学脊柱病研究所,上海200032 |
Institute of Spondylopathy,Shanghai University of TCM |
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| 施杞 |
SHI Qi |
上海中医药大学脊柱病研究所,上海200032 |
Institute of Spondylopathy,Shanghai University of TCM |
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| 周重建 |
ZHOU Chong-jian |
上海中医药大学脊柱病研究所,上海200032 |
Institute of Spondylopathy,Shanghai University of TCM |
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| 杨阳 |
YANG Yang |
上海中医药大学脊柱病研究所,上海200032 |
Institute of Spondylopathy,Shanghai University of TCM |
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| 侯宝兴 |
HOU Bao-xing |
上海中医药大学龙华医院伤骨科 |
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| 莫文 |
MUO Wen |
上海中医药大学龙华医院伤骨科 |
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| 期刊信息：《中国骨伤》2004年17卷，第4期，第198-200页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:国家自然科学基金(NO.39970917);国家中医药管理局基金(NO.00-01LQ11) |
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目的:比较检测风寒湿痹证型颈椎病动物模型中颈椎间盘Fas、bcl-2蛋白的表达;检测葛根汤、桂枝汤对风寒湿型颈椎病家兔颈椎间盘组织中Fas、bcl-2蛋白的调节作用。
方法:8月龄雄性新西兰白兔24只,随机分为正常对照组、风寒湿刺激组、葛根汤治疗组、桂枝汤治疗组。风寒湿刺激组温度6℃,湿度95%,风力6级,每日连续刺激4h,间断重复刺激128h;葛根汤、桂枝汤治疗组在风寒湿刺激组造模基础上服用。采用免疫组织化学ABC法检测椎间盘石蜡切片中Fas、bcl-2蛋白的表达情况。
结果:风寒湿刺激组同正常对照组比较,Fas表达均上调(P<0.01),葛根汤降低Fas表达,与风寒湿刺激组比较有显著性差异(P<0.01)。风寒湿刺激组同正常对照组比较,bcl-2表达均下降(P<0.05～0.01),葛根汤上调bcl-2表达,但与风寒湿刺激组比较没有显著性差异(P>0.05),桂枝汤对bcl-2的调节作用不明显。
结论:风寒湿刺激组同正常组对照比较,Fas表达均上调,bcl-2表达均下降,葛根汤降低Fas表达,上调bcl-2表达,发挥延缓椎间盘退变的作用,桂枝汤调节上述细胞因子的作用低于葛根汤。 |
| [关键词]:颈椎病 基因表达 葛根汤 桂枝汤 |
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| Expression of the Fas receptor,bcl-2 in the cervical intervertebral disc of the rabbit modified by the Guizhitang and Gegentang |
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Abstract:
Objective:To compare the expression of the Fas receptor,bcl-2 in the degenerative intervertebral disc of the rabbits cervical spondylosis model induced by Fenghanshi.To study the effects of the Guizhitang,Gegentang on the expression of the Fas receptor,bcl-2.
Methods:24 male rabbits were divided randomly into normal group,Fenghanshi group,Gegentang group and Guizhitang group.Fenghanshi model was created in 6 ℃ temperature,95% humidity,and 6 grade wind with 4 hours stimulation at each day for the total of 128 hours.Fenghanshi group was treated by Gegentang and Guizhitang.The expression of the Fas receptor,bcl-2 in the degenerative disc was detected with the immunohistochemistry ABC.
Results:The expression of the Fas receptor increase in the Fenghanshi model compare with the normal (P<0.01).Gegentang could regulate down the expression of Fas receptor in the fenghanshi model (P<0.01).On the other hand,the expression of the bcl-2 decrease in the Fenghanshi model compared with the normal (P<0.05～0.01).Gegentang could regulate up the expression of Fas receptor in the Fenghanshi model (P>0.01)
Conclusion: The expression of the Fas receptor can be regulated up and the bcl-2 regulated down in the Fenghanshi model compared with the normal.Gegentang can regulate down the expression of the Fas receptor and regulate up the bcl-2. |
| KEYWORDS:Cervical spondylopathy Gene expression Gegen decoction Guizhi decoction |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 刘梅,王拥军,施杞,周重建,杨阳,侯宝兴,莫文.葛根汤和桂枝汤调节椎间盘组织Fas、bcl-2蛋白表达的实验研究[J].中国骨伤,2004,17(4):198~200 |
| 英文格式: | LIU Mei,WANG Yong-jun,SHI Qi,ZHOU Chong-jian,YANG Yang,HOU Bao-xing,MUO Wen.Expression of the Fas receptor,bcl-2 in the cervical intervertebral disc of the rabbit modified by the Guizhitang and Gegentang[J].zhongguo gu shang / China J Orthop Trauma ,2004,17(4):198~200 |
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