| 实验性骨质疏松大鼠胫骨髁病理变化的图像分析研究 |
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Received:October 16, 2002
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| 作者 | Author | 单位 | Unit | E-Mail |
| 李莉 |
LI Li |
中国中医研究院骨伤科研究所,北京 100700 |
Institute of Orthopaedics and Traumatology, China Academy of TCM Beijing, 100700, China |
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| 张平 |
ZHANG Ping |
中国中医研究院骨伤科研究所,北京 100700 |
Institute of Orthopaedics and Traumatology, China Academy of TCM Beijing, 100700, China |
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| 沈乾一 |
SHEN Qianyi |
中国中医研究院骨伤科研究所,北京 100700 |
Institute of Orthopaedics and Traumatology, China Academy of TCM Beijing, 100700, China |
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| 韩凤岳 |
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中国中医研究院骨伤科研究所,北京 100700 |
Institute of Orthopaedics and Traumatology, China Academy of TCM Beijing, 100700, China |
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| 期刊信息：《中国骨伤》2003年16卷，第12期，第719-722页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
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目的:用骨计量学和去卵巢诱发骨质疏松模型的方法,观察大鼠胫骨髁组织病理学的基本变化。
方法:取40只成年纯系Wistar雌性大鼠,随机分为2组,造模组切除双侧卵巢3个月后,造成骨质疏松模型。对照组不切除卵巢,平行喂养。处死动物,取右侧胫骨上段,制备石蜡切片,行HE和奥新蓝染色。进行组织病理学观察、图像分析骨计量学研究和统计学处理。
结果:骨质疏松大鼠胫骨髁的小梁骨明显减少、变细、断裂形成钮扣状碎片、小梁骨的比面和比积减少、小梁骨和皮质骨内变性骨细胞和空化骨细胞陷凹数量增加,小梁骨的成骨细胞减少,而小梁骨钙化软骨轴的面积略有增加。以上数字化的指标与对照组大鼠的差异有显著性意义。
结论:骨质疏松时,骨的基本病理变化是小梁骨和皮质骨骨质破坏吸收、骨量减少、变性坏死骨细胞的数量增多和成骨细胞数量减少等。 |
| [关键词]:骨质疏松 胫骨 病理学 图像处理 计算机辅助 |
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| Micro-image analysis for pathological alteration in the tibial condyle of rat with experimental osteoporosis |
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Abstract:
Objective:To research on primary histopathologic alteration in the condyle tibiae of rat with osteoporosis induced by ovariotomy with micro-image analysis.
Methods:40 adult Wistar female rats were randomly divided into 2 groups:model group and control group. 3 months after bilateral ovariotomy, the rat models with osteoporosis were prepared. Rats in control group were not ovariotomy and feed same as model group. The rats were executed,and the tibiae were removed from the rats. The tibial condyles were prepared into paraffin sections and stained with HE and Alcian blue method. Histopathologic observation,micro-image analysis of os-teo-metrology,and statistic analysis were done.
Results:In the tibial condyles of model rat, the trabeculars became thin,and fracture forming into scraps as button, and trabecular amount, and trabecular specific surface and specific volume diminished distinctly,and number of metamorphic osteocytes and empty lacunae increased distinctly,and number of osteoblast decreased,area of calcified cartilage core in the trabecular was slightly augmented. There were significant differences between model and control groups.
Conclusion:In the tibial condyle of rat with osteoporosis, primary pathological alterations are that there are destroy and absorption,diminishing of bone mass in the trabecular and compact bone, and number of metamorphic and necrotic osteocyte increased, of osteoblast decreased,etc. |
| KEYWORDS:Osteoporosis Tibia Pathology Image interpretation, computer-assisted |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 李莉,张平,沈乾一,韩凤岳.实验性骨质疏松大鼠胫骨髁病理变化的图像分析研究[J].中国骨伤,2003,16(12):719~722 |
| 英文格式: | LI Li,ZHANG Ping,SHEN Qianyi.Micro-image analysis for pathological alteration in the tibial condyle of rat with experimental osteoporosis[J].zhongguo gu shang / China J Orthop Trauma ,2003,16(12):719~722 |
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