| Ⅱ型碳酸酐酶阻断剂对体外破骨细胞的抑制作用 |
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Received:November 18, 2002 Revised:January 20, 2003
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| 期刊信息：《中国骨伤》2003年16卷，第7期，第409-411页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
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目的:研究Ⅱ型碳酸酐酶阻断剂对体外破骨细胞骨吸收过程的影响。
方法:由一日龄SD大鼠四肢长骨分离破骨细胞并接种于象牙骨片上共同培养,在培养液中分别加入不同浓度的Ⅱ型碳酸酐酶阻断剂乙酰唑胺,观察3天和9天时破骨细胞骨吸收能力的变化,从而证实Ⅱ型碳酸酐酶对破骨细胞性骨吸收的影响。
结果 10-6M乙酰唑胺使骨吸收陷窝数和吸收面积均显著减少,浓度为10-8M时只对骨吸收面积有显著抑制作用。
结论:Ⅱ型碳酸酐酶阻断剂通过调整破骨细胞内pH值影响破骨细胞分化、成熟及活化进而影响骨吸收。可以对老年性骨质疏松症疾病的治疗发挥早期、连续、持久作用。 |
| [关键词]:骨质疏松 组织学 碳酸脱水酶 细胞培养 |
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| Inhibition of osetoclasts in vitro by carbonic anhydrase Ⅱ inhibitor |
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Abstract:
Objective:To study effects of carbonic anhydrase Ⅱ inhibitor on bone resorption process of osteoclasts in vitro
Methods:The osteoclasts from long bones of SD rats aged one-day were isolated and seeded in ivory bones for culture. Carbonic anhydrase Ⅱ inhibitor-acetazolamide with different concentrations was added to culture media.Changes of the bone resorption ability of the osteoclasts were observed in 3rd and 9th days.The effects of carbonic anhydrase Ⅱ on osteoclast bone resorption were comfirmed.
Results:Both the pit numbers and the pit areas were significantly reduced after using acetazolamide at the concentration of 10-6 M,while only the pit areas were reduced at the concentration of 10-8 M.
Conclusion:Carbonic anhydrase Ⅱ inhibitor played a major role in osteoclast differentiation,maturity and activation by effecting the steady state of pH.Thus,it affected the bone resorption and could play an earlier,successive,continual role in treatment of senile osteoporosis. |
| KEYWORDS:Osteoporosis Histology Carbonate dehydratase Cell culture |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 王文军,李建军,李小春,冷向阳.Ⅱ型碳酸酐酶阻断剂对体外破骨细胞的抑制作用[J].中国骨伤,2003,16(7):409~411 |
| 英文格式: | WANG Wenjun,LI Jianjun,LI Xiaochun,LENG Xiangyang.Inhibition of osetoclasts in vitro by carbonic anhydrase Ⅱ inhibitor[J].zhongguo gu shang / China J Orthop Trauma ,2003,16(7):409~411 |
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