| 家兔骨折愈合过程中软骨细胞凋亡的透射电镜观察 |
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Received:August 16, 2002
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| 期刊信息：《中国骨伤》2003年16卷，第4期，第217-219页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
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目的:观察骨折愈合中软骨细胞在软骨化骨时的死亡方式及软骨细胞的其它可能结局。
方法:采用家兔左桡骨标准骨折,在软骨骨痂产生的不同时期、不同部位取材,常规制作透射电镜观察标本。
结果 :①软骨细胞生长、消亡过程可分为3个阶段:成软骨细胞、肥大软骨细胞、凋亡软骨细胞。软骨细胞凋亡表现为核固缩和凋亡小体形成,但细胞器不被破坏。最后,软骨细胞崩解,凋亡小体散落于软骨基质中。②软骨化骨过程中只是部分软骨细胞凋亡;另一部分软骨细胞并不出现凋亡,直接转变为骨细胞。
结论:①骨折愈合时发生的软骨化骨,是机体通过激活软骨细胞死亡程序,以细胞凋亡达到新老细胞交替,实现化骨。②由肥大软骨细胞组成的软骨骨痂,能直接转变为编织骨并骨化,支持软骨细胞能直接转变为骨细胞的理论。 |
| [关键词]:骨折愈合 软骨细胞 凋亡 电子显微镜检查,透射 |
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| Transmission electron microscopy for the chondrocyte apoptosis in rabbit’s fracture healing |
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Abstract:
Objective:To observe the dead manner and other possible outcomes of chondrocytes in the healing process of fracture
Methods:The standardized fracture of left radial bone was created in rabbits. The cartilage callus at the fracture site were taken at the different healing time and different position,and observed under the transmission electron microscope.
Results:The Results of the study indicated that there were three stages of chondrocyte changes at the healing process: chondroblasts, hypertrophic chondrocytes and apoptotic chondrocytes. The apoptosis of chondrocytes showed nucleus shrink and apoptotic bodies,but no rupture of or-ganelles. Finally,the apoptotic chondrocytes broke down and many apoptotic bodies were in cartilage matrix. Also it was found that not all of chondrocytes appeared apoptosis in the endochondrial ossification process. The chondrocytes without apoptosis might directly transform into osteocytes.
Conclusion:① In fracture healing process, the endochondrial ossification was mediated by chondrocyte apoptosis. ②The cartilage callus made of hypertrophic chondrocytes can directly transform into the woven bones. |
| KEYWORDS:Fractures healing Chondrocyte Apoptosis Electron microscopy, transmission |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 严蕊琳,马时荣,王桂华,王祖武.家兔骨折愈合过程中软骨细胞凋亡的透射电镜观察[J].中国骨伤,2003,16(4):217~219 |
| 英文格式: | YAN Ruilin,MA Shirong,WANG Guihua.Transmission electron microscopy for the chondrocyte apoptosis in rabbit’s fracture healing[J].zhongguo gu shang / China J Orthop Trauma ,2003,16(4):217~219 |
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