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补肾活血方对离体胎鼠颅骨成骨细胞增殖的影响

Hits: 1793 Download times: 1160 Received:August 26, 2002 Revised:January 02, 2003

作者	Author	单位	Unit	E-Mail
汤耿民	TANG Gengmin	湖北省中医药研究院,湖北武汉 430074	Hubei Academy of Traditional Chinese Medicinal and Pharmacy, Hubei Wuhan, 430074, China
沈霖	SHEN Lin	华中科技大学同济医学院附属协和医院
肖明英	XIAO Mingying	湖北省中医药研究院,湖北武汉 430074	Hubei Academy of Traditional Chinese Medicinal and Pharmacy, Hubei Wuhan, 430074, China

期刊信息：《中国骨伤》2003年16卷，第4期，第211-212页

DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz

基金项目:湖北省卫生科研基金(W9721);湖北省自然科学基金(99J136)

目的:探讨补肾活血法对成骨细胞代谢的影响。

方法:胎鼠颅骨成骨细胞培养于含有0,10,100,1000,5 000μg.ml^-15种浓度的补肾活血方药液培养基中72h后,分别采用噻唑篮比色法和胸腺嘧啶核苷渗入法检测成骨细胞增殖、分化情况,并与具有促细胞增殖作用的成纤维细胞生长因子作对照。

结果 :补肾活血方药液以剂量依赖方式促进成骨细胞的增殖,与对照组相比,100 g.d-‘药液即可促进成骨细胞增殖,并在1000μg.ml^-1浓度时达到最大效应（P<0.05,P<0.01）。

结论:补肾活血方具有促进骨形成作用。

[关键词]:成骨细胞动物,实验中草药

Effects of Chinese medicine Bushenhuoxue on the proliferation of fetal rat calvarial osteoblast in vitro

Abstract:

Objective:To investigate the effects of Bushenghuoxue on osteoblastic cell metabolism

Methods:Fetal rat calvarial osteoblastic cell were cultured in the medium containing Bushenghuoxue with five different concentrations,0,10,100,1 000,and 5 000μg·ml-1 ,and incubated for 72 hours.Calorimetric MMT,3H-thymidine incorporation were used to determine the condition of cell proliferation and differentiation in vitro.

Results:Bushenghuoxue with 100 μg·ml-1 might promoted the proliferation of osteoblastic cells. Its effects was concentration-dependent. 1000 μg·ml-1 may reach the highest effect.

Conclusion:Bushenghuoxue can promote the bone formation.

KEYWORDS:Osteoblasts Animals, laboratory Drugs, Chinese herbal

引用本文，请按以下格式著录参考文献：

中文格式:	汤耿民,沈霖,肖明英.补肾活血方对离体胎鼠颅骨成骨细胞增殖的影响[J].中国骨伤,2003,16(4):211~212
英文格式:	TANG Gengmin,SHEN Lin,XIAO Mingying.Effects of Chinese medicine Bushenhuoxue on the proliferation of fetal rat calvarial osteoblast in vitro[J].zhongguo gu shang / China J Orthop Trauma ,2003,16(4):211~212

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