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复合型完全脱蛋白骨的细胞相容性研究

Hits: 1938 Download times: 1225 Received:November 26, 2001

作者	Author	单位	Unit	E-Mail
李彦林	LI Yan-lin	昆明医学院第一附属医院骨科, 云南昆明 650032	The First Affiliated Hospital of Kunming Medical College Yunnan Kunming,650032
杨志明	YANG Zhi-ming	华西医科大学附一院骨科
韩睿	HAN Rui	昆明医学院第一附属医院骨科, 云南昆明 650032	The First Affiliated Hospital of Kunming Medical College Yunnan Kunming,650032
李世和		昆明医学院第一附属医院骨科, 云南昆明 650032	The First Affiliated Hospital of Kunming Medical College Yunnan Kunming,650032
曾才铭		昆明医学院第一附属医院骨科, 云南昆明 650032	The First Affiliated Hospital of Kunming Medical College Yunnan Kunming,650032

期刊信息：《中国骨伤》2002年15卷，第10期，第597-599页

DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz

基金项目:云南省自然科学基金资助项目(2000C0010R)

目的:探讨复合型完全脱蛋白骨的细胞相容性。

方法:将猪肋骨用理化方法制得复合型完全脱蛋白骨(CFDB),对CFDB理化性能进行检测,又将其与人胎骨膜成骨细胞体外复合培养,以了解其细胞相容性及细胞毒性。

结果:CFDB的主要成份为羟基磷灰石,蛋白质含量几乎为零,仍具有原骨组织骨盐支架的三维多孔网状孔隙结构系统,对复合培养的人胎骨膜成骨细胞的形态特征、ALP活性、细胞周期、倍体水平无有害影响。

结论:CFDB具有原骨组织的网状孔隙结构系统,具有良好的细胞相容性,可望用于成骨细胞的支架材料。

[关键词]:成骨细胞细胞培养复合型完全脱蛋白骨

Cellular compatibility study of composite fully deproteinised bone

Abstract:

Objective:To study the cellular compatibility of composite fully deproteinised bone(CFDB)

Methods:CFDB was processed by physical and chemical methods with pig ribs and its properties were examined, Then its cellular compatibility and cytotoxicity were examined by osteoblasts culture in vitro.

Results:The main ingredients of CFDB was hydroxyapatite;it hardly had any protein,and it had natural pore system which possessed pore network system of original bone. It has no bad affect on cell feature,alkaline phosphatase activity and cell cycle of human embryonic periosteal-derived osteoblasts composited with CFDB.

Conclusion:CFDB possessed original bone natural pore system and well cellular compatibility,and it could be used as scalfolds of osteoblasts.

KEYWORDS:Osteoblast Cell culture Composite fully deproteinised bone

引用本文，请按以下格式著录参考文献：

中文格式:	李彦林,杨志明,韩睿,李世和,曾才铭.复合型完全脱蛋白骨的细胞相容性研究[J].中国骨伤,2002,15(10):597~599
英文格式:	LI Yan-lin,YANG Zhi-ming,HAN Rui.Cellular compatibility study of composite fully deproteinised bone[J].zhongguo gu shang / China J Orthop Trauma ,2002,15(10):597~599

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