| 风寒湿刺激对家兔颈椎间盘MMP-1、MMP-3活性的影响 |
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Received:September 25, 2001
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| 作者 | Author | 单位 | Unit | E-Mail |
| 施杞 |
SHI Qi |
上海中医药大学附属龙华医院,上海200032 |
Longhua Hospital,Shanghai University of TCM Shanghai,200032 |
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| 王拥军 |
WANG Yong jun |
上海中医药大学附属龙华医院,上海200032 |
Longhua Hospital,Shanghai University of TCM Shanghai,200032 |
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| 吴士良 |
WU Shi liang |
苏州大学医学院,江苏苏州 |
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| 葛京化 |
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上海中医药大学附属龙华医院,上海200032 |
Longhua Hospital,Shanghai University of TCM Shanghai,200032 |
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| 杨阳 |
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上海中医药大学附属龙华医院,上海200032 |
Longhua Hospital,Shanghai University of TCM Shanghai,200032 |
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| 刘梅 |
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上海中医药大学附属龙华医院,上海200032 |
Longhua Hospital,Shanghai University of TCM Shanghai,200032 |
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| 周重建 |
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上海中医药大学附属龙华医院,上海200032 |
Longhua Hospital,Shanghai University of TCM Shanghai,200032 |
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| 期刊信息：《中国骨伤》2002年15卷，第6期，第340-342页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:国家自然科学基金项目(No.39970917) |
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目的:在建立不同强度风寒湿痹证型颈椎病动物模型的基础上,检测实验家兔颈椎间盘组织中胶原酶(MMP1)、中性蛋白多糖酶(MMP3)活性变化。
方法:8月龄雄性新西兰白兔24只,随机分为正常对照组和轻度、中度、重度刺激组。采用刺激条件是温度5℃±0.5℃,湿度100%,风力6级,按轻、中、重度刺激组的不同要求,分别给予32、64和128小时的间断重复刺激,每日刺激4小时。分别采用3MH-Ⅰ型胶原蛋白法和免疫一步夹心法检测MMP-1、MMP-3活性。
结果:各个模型组退变指数都有不同程度上升,重度刺激组较正常对照组有统计学差异(P<0.05)。模型组与对照组比较,中、重度刺激组MMP-1和MMP-3活性升高明显(P<0.01),轻度刺激组变化不明显;模型组之间比较,中、重度刺激组MMP-1、MMP-3活性明显高于轻度刺激组(P<0.01)。
结论:中、重度风寒湿刺激家兔颈椎间盘退变后使MMP1和MMP3活性升高,加速椎间盘细胞外基质降解,导致颈椎间盘退变。 |
| [关键词]:颈椎病 痹证 胶原酶 中性蛋白多糖酶 |
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| Effect on activity change of MMP-1,MMP-3 of cervical intervertebral discs of rabbits by irritancy of Wind-Cold-Dampness factors |
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Abstract:
Objective:To establish cervical spondylosis animal models of Wind Cold Dampness arthralgia syndrome type.The morphologic state of the discs of the rabbits was observed with light microscope,and the activity of metalloproteinase 1,metalloproteinase 3 in the discs was assayed with biochemical.
Methods:Twenty four 8 months old rabbits were randomly allocated into four groups of the control,light,medium and heavy irritancy groups with 6 animals in each.The basic condition of Wind Cold Dampness is 6 class force of wind, 5℃±0.5℃ and 100% humidity.32,64,128 hours of continuous irritancy were applied on light,medium and heavy irritancy group respectively.The daily period of irritancy was 4 hour.
Results:The degree index number of intervertebral discs degeneration was elevated in every irritancy group,and in the heavy irritancy group the increase was significantly higher(P<0.05).MMP 1 and MMP 3 activities of cervical intervertebral discs in middle and heavy irritancy group,MMP 1 and MMP 3 activity in medium and heavy irritancy group were significantly higher than that in the control group respectively(P<0.01).
Conclusion:MMP 1 and MMP 3 activity were increased by medium and heavy Wind Cold Dampness irritancy.Acceleration of degradation of extracelluar matrix of cervical intervertebral discs resulted in degeneration of cervical intervertebral discs. |
| KEYWORDS:Cervical syndrome Arthralgia syndrome Metalloproteinases Ⅰ Metalloproteinases Ⅲ |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 施杞,王拥军,吴士良,葛京化,杨阳,刘梅,周重建.风寒湿刺激对家兔颈椎间盘MMP-1、MMP-3活性的影响[J].中国骨伤,2002,15(6):340~342 |
| 英文格式: | SHI Qi,WANG Yong jun,WU Shi liang.Effect on activity change of MMP-1,MMP-3 of cervical intervertebral discs of rabbits by irritancy of Wind-Cold-Dampness factors[J].zhongguo gu shang / China J Orthop Trauma ,2002,15(6):340~342 |
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