| 补肾法对卵巢切除大鼠骨组织白细胞介素-6mRNA表达的影响 |
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Received:October 15, 2001
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| 作者 | Author | 单位 | Unit | E-Mail |
| 沈霖 |
SHEN Lin |
华中科技大学同济医学院附属协和医院,湖北武汉430022 |
Xiehe Affiliated Hopspital, Tongji Medical College, Huazhong University of Science and Technology Hubei Wuhan ,430022 |
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| 杜靖远 |
DU Jing-yuan |
华中科技大学同济医学院附属协和医院,湖北武汉430022 |
Xiehe Affiliated Hopspital, Tongji Medical College, Huazhong University of Science and Technology Hubei Wuhan ,430022 |
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| 涂意辉 |
TU Yi-hui |
华中科技大学同济医学院附属协和医院,湖北武汉430022 |
Xiehe Affiliated Hopspital, Tongji Medical College, Huazhong University of Science and Technology Hubei Wuhan ,430022 |
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| 杨艳萍 |
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华中科技大学同济医学院附属协和医院,湖北武汉430022 |
Xiehe Affiliated Hopspital, Tongji Medical College, Huazhong University of Science and Technology Hubei Wuhan ,430022 |
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| 谢晶 |
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华中科技大学同济医学院附属协和医院,湖北武汉430022 |
Xiehe Affiliated Hopspital, Tongji Medical College, Huazhong University of Science and Technology Hubei Wuhan ,430022 |
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| 周丕琪 |
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华中科技大学同济医学院附属协和医院,湖北武汉430022 |
Xiehe Affiliated Hopspital, Tongji Medical College, Huazhong University of Science and Technology Hubei Wuhan ,430022 |
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| 期刊信息：《中国骨伤》2002年15卷，第4期，第211-213页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:国家中医药管理局课题(编号2000-J-B-19) |
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目的:探讨补肾法治疗绝经后骨质疏松症的分子作用环节。
方法:以卵巢切除大鼠作为骨质疏松实验模型,通过斑点杂交技术,在InRNA水平分析模型组、密骨片组及假手术组白细胞介素-6(IL-6)表达。通过原位杂交技术,研究了 IL-6 MA在骨组织细胞的分布。
结果: 模型组大鼠 IL-6InRNA表达较假手术组明显升高(P<0.01);而密骨片组治疗后 IL6 MA表达明显下降(P<0.01)。IL6 mRNA主要在成骨细胞、骨衬里细胞和骨细胞胞浆内表达。
结论:IL6在骨质疏松骨丢失过程中起重要作用;补肾法抗骨质疏松疗效部分是通过降低IL6产生而介导的。 |
| [关键词]:骨质疏松,绝经后 卵巢切除术 中医现代化 |
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| Effects of kidney invigoration method on expression of interleukin-6 mRNA in bone of ovariectomized rats |
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Abstract:
Objective:investigate the molecular links of the treatment with kidney invigoration method on postmenopausal osteoporosis in vivo.
Methods:Adult ovariectomized rats were used in this study as the model of osteoporosis. Expression of interleukin-6(IL-6)in bone tissue of sham operate(Sham) .ovariecto-my(OVX)and ovariectomy with Migu tablet(OVX + MGT)rats was analysed at mRNA level using dot blot hybridization. The distribution of IL-6 mRAN was studied by in situ hybridization.
Results:The expression of IL-6 was higher in bone tissue of OVX than that of Sham group(P<0.01) .while treated with MGT.the ex-pression of IL-6 significantly declined compared with that in OVX group(P<0.01).The in situ hybridization showed that 11-6 mRNA was mainly expressed in osteoblasts, lining cell and osteocytes.
Conclusion: These date indicate that IL-6 may pky an important role in bone loss of osteoporosis. Suppression for IL-6 production was involved in the mechanism of kidney invigoration in the treatment of osteoporosis. |
| KEYWORDS:Osteoporosis,postmenopausal Ovariectomy Moderation of TCM |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 沈霖,杜靖远,涂意辉,杨艳萍,谢晶,周丕琪.补肾法对卵巢切除大鼠骨组织白细胞介素-6mRNA表达的影响[J].中国骨伤,2002,15(4):211~213 |
| 英文格式: | SHEN Lin,DU Jing-yuan,TU Yi-hui.Effects of kidney invigoration method on expression of interleukin-6 mRNA in bone of ovariectomized rats[J].zhongguo gu shang / China J Orthop Trauma ,2002,15(4):211~213 |
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