| 尼莫地平对体外培养成骨细胞的影响 |
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Received:November 08, 2000 Revised:January 20, 2001
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| 作者 | Author | 单位 | Unit | E-Mail |
| 朱建民 |
ZHU Jian-min |
上海市第八人民医院,上海200032 |
The 8th People’s Hospital of Shanghai,Shanghai,200032 |
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| 方浩 |
FANG Hao |
上海市第八人民医院,上海200032 |
The 8th People’s Hospital of Shanghai,Shanghai,200032 |
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| 陈新刚 |
CHEN Xin-gang |
上海市第八人民医院,上海200032 |
The 8th People’s Hospital of Shanghai,Shanghai,200032 |
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| 曾明 |
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上海市第八人民医院,上海200032 |
The 8th People’s Hospital of Shanghai,Shanghai,200032 |
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| 金蔚芳 |
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复旦大学医学院骨代谢研究室 |
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| 王洪复 |
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复旦大学医学院骨代谢研究室 |
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| 期刊信息：《中国骨伤》2001年14卷，第12期，第730-732页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:上海市徐汇区卫生局科研基金资助(编号:99012) |
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目的：观察尼莫地平对体外培养成骨细胞的作用。
方法：在新生SD大鼠头颅骨第2继代成骨细胞(OB2)培养液中分别加入不同浓度(10-1～10-9g/L)尼莫地平,分别观察OB2的增殖功能(用波长570nm处OD值表示),分化功能(用碱性磷酸酶ALP活性表示)和矿化功能(用矿化结节数量/视野表示).
结果：增殖功能OD值为012±001～041±004;ALP活性为009±001U/mg蛋白质；矿化结节数量/视野为13±09个。
结论：与对照组比较,尼莫地平各浓度对OB2的增殖、分化和矿化功能作用各异。当尼莫地平浓度为10-6g/L时,对OB2的增殖和分化功能具有刺激作用,对OB2的矿化功能具有显着的抑制作用；当浓度升高(10-2g/L)时,对OB2的增殖功能具有明显的抑制作用；当浓度降低(10－8g/L)时，对OB2的增殖功能失去作用。 |
| [关键词]:钙通道阻滞药 成骨细胞 组织培养 |
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| The effect of Nimodipine on culture of osteoblasts in vitro |
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Abstract:
Objective:To observe the effect of nimodipine on culture of osteoblasts in vitro
Methods: The culture medium with different nimodipine concentrations(10-1 ～10-9 g/L)and the second generation osteoblats(OB2)from the skull of new born SD rats were mixed for the observation respectively about the proliferation(OD value at wavelength 570nm),the differentiation(ALP activity)and the mineralization(mineralized nodes/field of vision)of OB2.
Results:The proliferation function was 0.12±0.01～0 41±0.04 OD value(mean and standard deviation);the ALP activity(mean and standard deviation)was 0.09±0.01U/mg protein and the mineralized nodes(mean and standard deviation)was 1.3±0.9.
Conclusion: In comparison with control groups,the proliferation and differentiation of the OB2 were remarkably increased by 10-6 g/L nimodipine concentration and the mineralization was significantly inhibited.The proliferation of the OB2 was significantly inhibited with the increase of nimodopine concentration to 10-2 g/L,the proliferation was of no effect when the concentration of nimodipine was decreased to 10-8 g/L. |
| KEYWORDS:Calcium channel blockers Osteoblasts Tissue culture |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 朱建民,方浩,陈新刚,曾明,金蔚芳,王洪复.尼莫地平对体外培养成骨细胞的影响[J].中国骨伤,2001,14(12):730~732 |
| 英文格式: | ZHU Jian-min,FANG Hao,CHEN Xin-gang.The effect of Nimodipine on culture of osteoblasts in vitro[J].zhongguo gu shang / China J Orthop Trauma ,2001,14(12):730~732 |
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