| 猪骨-rhBMP-2复合重组人工骨的研制及临床应用研究 |
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Received:October 17, 2000 Revised:January 10, 2001
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| 作者 | Author | 单位 | Unit | E-Mail |
| 李万民 |
LI Wan-min |
濉溪县医院,安徽濉溪235100 |
Suixi Hospital Anhui Suixi,235100 |
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| 赵明 |
ZHAO Ming |
中国军事医学科学院基础医学研究所 |
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| 徐杰 |
XU Jie |
濉溪县医院,安徽濉溪235100 |
Suixi Hospital Anhui Suixi,235100 |
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| 刘农乐 |
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中国军事医学科学院基础医学研究所 |
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| 周丙怀 |
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濉溪县医院,安徽濉溪235100 |
Suixi Hospital Anhui Suixi,235100 |
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| 王会信 |
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中国军事医学科学院基础医学研究所 |
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| 马立志 |
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濉溪县医院,安徽濉溪235100 |
Suixi Hospital Anhui Suixi,235100 |
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| 赵怀川 |
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濉溪县医院,安徽濉溪235100 |
Suixi Hospital Anhui Suixi,235100 |
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| 苏维成 |
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濉溪县医院,安徽濉溪235100 |
Suixi Hospital Anhui Suixi,235100 |
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| 期刊信息：《中国骨伤》2001年14卷，第12期，第719-721页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
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目的：选择猪骨作为rhBMP2的载体,经去抗原处理,复合重组人工骨,观察该种人工骨临床应用骨缺损修复,促进骨折愈合及诱导成骨的能力。
方法：选择猪骨经透析冻干法脱脂、脱蛋白及部分脱钙处理后与成品rhBMP2按一定比例组成具有生物活性的猪骨-rhBMP2复合人工骨,先行动物模型实验,随后以自愿随机的原则,临床应用32例,术后1,4,8,12周进行X线检查,观察临床表现,功能恢复情况。
结果：临床32例,34块骨,35处植入。随访31例,随访率96.7%,最长24个月,最短3个月；骨的愈合及缺损修复满意,临床无不良反应。
结论：经透析冻干法处理的猪骨能成为rhBMP2的理想载体,与rhBMP2复合后具有良好的成骨诱导活性,无免疫排斥反应,具有广泛的临床应用前景。 |
| [关键词]:骨移植 移植,异种 基因 |
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| The preparation and clinical studies of artificial bone compounded and recombinated with pig bone rhBMP-2 |
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Abstract:
Objective:Pig bones eliminated of the antigens were used for rhBMP-2 carriers to compound and reconstruct artificial bones.The ability of this artificial bone in repairing bone defects,promoting fracture healing and inducing bone formation were observed
Methods: Pig bones with the fat separated albumen eliminated together with parts of calcium by means of dialysis lyophilization and then composed biology active artificial pig bones-rhBMP-2 with rhBMP-2 were composed in a certain proportion.The bones were applied in animal model experimental studies and then used clinically in 32 cases at random based on voluntary principle.Clinical manifestation and function recovery were observed by X-rays examination in 1,4,8 and 12 weeks after operation.
Results:32 cases of 34 bones with 35 insertion were used.96.7% of the 31 cases were followed up from 3～24 months.The bone repair and the patients recovery were satisfactory and there was no side reaction.
Conclusion: The processed pig bone is an ideal carriers of rhBMP-2 and has the action of inducing bone formation,without immunological rejection and it is of extensive prospects in clinical application. |
| KEYWORDS:Bone transplantation Transplantation heterologous Genes |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 李万民,赵明,徐杰,刘农乐,周丙怀,王会信,马立志,赵怀川,苏维成.猪骨-rhBMP-2复合重组人工骨的研制及临床应用研究[J].中国骨伤,2001,14(12):719~721 |
| 英文格式: | LI Wan-min,ZHAO Ming,XU Jie.The preparation and clinical studies of artificial bone compounded and recombinated with pig bone rhBMP-2[J].zhongguo gu shang / China J Orthop Trauma ,2001,14(12):719~721 |
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