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IGF-Ⅰ对培养兔关节软骨细胞作用的实验研究
Hits: 1703   Download times: 1323   Received:December 24, 1999  Revised:March 03, 2000  
作者Author单位UnitE-Mail
蓝旭 LAN Xu 兰州军区总医院骨科研究所,甘肃兰州730050 General Hospital of Lanzhou Military Area Gansu Lanzhou,730050  
刘雪梅 LIU Xue mei 兰州军区总医院骨科研究所,甘肃兰州730050 General Hospital of Lanzhou Military Area Gansu Lanzhou,730050  
葛宝丰 GE Bao Feng 兰州军区总医院骨科研究所,甘肃兰州730050 General Hospital of Lanzhou Military Area Gansu Lanzhou,730050  
许建中 第三军医大学西南医院,重庆  
期刊信息:《中国骨伤》2001年14卷,第6期,第341-342页
DOI:doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz


目的:了解胰岛素样生长因子Ⅰ(IGFⅠ)对培养关节软骨细胞增殖及代谢的影响。

方法:体外单层培养兔关节软骨细胞,实验组以10,100,200ng/mlIGFⅠ作用细胞2、4、6天,对照组不加IGFⅠ作用培养细胞。检测细胞DNA及基质中糖醛酸含量。用流式细胞仪分析在100ng/mlIGFⅠ作用下细胞周期亚时相。

结果:IGFⅠ在10~100ng/ml浓度范围内,对培养软骨细胞增殖和代谢有促进作用,以100ng/ml浓度作用效果最佳。细胞周期分析,实验组DNA合成前期(G1期)较对照组缩短。

结论:IGFⅠ促进软骨细胞的增殖与代谢,且通过缩短细胞G1期而缩短细胞周期。
[关键词]:胰岛素样生长因子Ⅰ  关节疾病  软骨
 
Effects of IGF-Ⅰ on proliferation and metabolism of cultured rabbit artiular chondrocytes
Abstract:

Objective:To investigate the effect of insulin like growth factor Ⅰ(IGF Ⅰ) on proliferation and metabolism of cultured articular chondrocytes.

Methods:The monolayer chondrocytes obtained from rabbit were cultured.The experimental group was treated with IGF Ⅰ at doses ranging from 10,100,200ng/ml at 2,4,and 6 days while the control group was cultured without IGF Ⅰ.The cell DNA content and matrical glucuronic acid were maesured.The cell cycle of chondrocytes treaed with IGF Ⅰ at 100ng/ml was determined by flow cytometer.

Results:IGF Ⅰ stimulated proliferation and metabolism of chondrocytes at doses ranging from 10 to 100ng/ml.Maximal effect of stimulation occurred at 100ng/ml after treatment.The time of DNA synthesis(G1) was shorter in experimental group than in control group.]

Conclusion:IGF Ⅰ stimulated proliferation and metabolism of chondrocytes and shortened the time needed for G1 phase.
KEYWORDS:Insulin  like  growth  factor    Joint  diseases  Cartilage
 
引用本文,请按以下格式著录参考文献:
中文格式:蓝旭,刘雪梅,葛宝丰,许建中.IGF-Ⅰ对培养兔关节软骨细胞作用的实验研究[J].中国骨伤,2001,14(6):341~342
英文格式:LAN Xu,LIU Xue mei,GE Bao Feng.Effects of IGF-Ⅰ on proliferation and metabolism of cultured rabbit artiular chondrocytes[J].zhongguo gu shang / China J Orthop Trauma ,2001,14(6):341~342
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