| 用含药血清方法观察补肾益精方对破骨细胞功能的影响 |
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Received:September 08, 1999 Revised:February 18, 2000
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| 作者 | Author | 单位 | Unit | E-Mail |
| 詹红生 |
ZHAN Hong sheng |
上海中医药大学附属曙光医院,上海200021 |
Shuguang Affiliated Hospital,Shanghai University of TCM Sha nghai,200021 |
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| 赵咏芳 |
ZHAO Yong fang |
上海市中医药研究院骨伤科研究所,上海 |
Shuguang Affiliated Hospital,Shanghai University of TCM Sha nghai,200021 |
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| 石印玉 |
SHI Yin yu |
上海中医药大学附属曙光医院,上海200021 |
Shuguang Affiliated Hospital,Shanghai University of TCM Sha nghai,200021 |
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| 高建军 |
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上海医科大学,上海 |
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| 沈培芝 |
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上海市中医药研究院骨伤科研究所,上海 |
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| 徐宇 |
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上海市中医药研究院骨伤科研究所,上海 |
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| 期刊信息：《中国骨伤》2001年14卷，第3期，第145-147页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:国家九五攻关资助项目(No.969060905) |
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目的;探讨补肾益精方含药血清的制备条件及其对体外培养破骨细胞骨吸收功能的影响。
方法;取体重270±20g的SD大鼠,雌雄各半,每组10只,同等条件下制备补肾益精方含药血清和对照血清,分别观察不同采血时间、不同喂药时间、不同灌胃剂量和血清添加量对骨吸收陷窝数量的影响。
结果;末次灌胃后1h采血组的骨吸收陷窝数明显少于其他各组(P<0.05);灌胃1d和3d、7d组比较其含药血清的药效无差异,而与对照血清差异显著；随着灌胃剂量和含药血清添加量的提高,其骨吸收陷窝数明显减少。
结论;补肾益精方含药血清对破骨细胞骨吸收功能具有明显的抑制作用；补肾益精方用于破骨细胞药效观察的大鼠含药血清制备条件为等效剂量连续两次(间隔2h)灌胃,末次灌胃后1h采血,其血清添加浓度为30%. |
| [关键词]:中草药 破骨细胞 血清学 |
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| The effects of Bushenyijing(invi gorating the kidney and supplementing th e essence of life)prescription on osteoc lasts resorption using drug sera method |
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Abstract:
Objective;To explore the preparation method of dru g sera of Bushenyijing Fang(BSYJF)(a pre scription of traditional Chinese medicin e)for invigorating the renal function an d the supplement of essence of life.Its effects on osteoclasts resorption tested in vitro were studied.
Methods;20 SD rats with average weight of 270±20 g,female and male in equal numbers,were divided into two groups randomly;the con trol group using sera and the group usin g sera including BSYJF.The effects of th e two kind of sera on the quantity of bo ne resorption lacuna were observed at di fferent blood sampling times,different m edicine administration times,different c onditions of medicine dosage and sera ad dition dosage.
Results;The quantity of bone resorption lacuna a t one hour after last time of administra tion of medicine was much more smaller t han that of other groups(P<0.05).Durg effects showed no difference at 1st,3rd and 7th days after medication,bu t significient difference was seen in co mparison with the control sera.With medi cine dose and sera addition dose increas ing,the bone resorption lacuna decreased obviously.
Conclusion;The drug sera of BSYJF significantly inh ibited osteoclast resorption.The prepara tion conditions of drug sera of BSYJF we re as follows;giving medicine of equival ent dose twice between 2 hours,sampling blood at one hour after last medication and adding sera to the concentration of 30%。 |
| KEYWORDS:Chinese herbal medicine Osteoclasts Sero logy |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 詹红生,赵咏芳,石印玉,高建军,沈培芝,徐宇.用含药血清方法观察补肾益精方对破骨细胞功能的影响[J].中国骨伤,2001,14(3):145~147 |
| 英文格式: | ZHAN Hong sheng,ZHAO Yong fang,SHI Yin yu.The effects of Bushenyijing(invi gorating the kidney and supplementing th e essence of life)prescription on osteoc lasts resorption using drug sera method[J].zhongguo gu shang / China J Orthop Trauma ,2001,14(3):145~147 |
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