| 内源性BMP、TGF-β在骨修复中的作用 |
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Received:January 15, 1998 Revised:April 22, 1998
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| 期刊信息：《中国骨伤》2000年13卷，第7期，第396-397页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
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目的:研究内源性骨形态发生蛋白(BMP)、β转化生长因子(TGF-β)的来源、分泌规律以及对骨修复的作用。
方法:家兔36只,每只动物左侧胫骨为对照侧,环形切除胫骨中段骨膜长3cm,并于内侧行部分骨皮质剥脱术,长26cm,厚1mm,不暴露骨髓腔。右侧为实验侧,以同法处理后,辅以钻孔达骨髓腔。术后3天及1、2、4、6、8周分别随机处死6只动物,取双侧胫骨,制作5μm厚的组织切片,进行BMP、TGF-βⅢ免疫组化染色。
结果:BMP、TGF-βⅢ免疫组化染色显示,对照侧染色阴性,始终无骨痂形成。实验侧术后3天染色呈阳性反应;术后1周内源性BMP、TGF-βⅢ浓度达高峰,成骨能力增强;术后6周阳性细胞消失,但随着骨重建过程增强,骨基质染色较深。
结论:骨损伤后内源性BMP、TGF-βⅢ呈阶段性分泌,且对骨损伤的修复起着重要作用。 |
| [关键词]:骨修复 骨形态发生蛋白 β-转化生长因子 免疫组织化学 |
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| The effect of endogenous BMP and TGF-β on bone repair |
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Abstract:
Objective:To explore the source and secretive regularity of endogenous bone morphogenetic protein(BMP)and Transforming growth factor β(TGF-β),as well as the effect of endogenous BMP and TGF β on bone repair.[HT5”H]
Methods:[HT5”SS]Periosteum in midpiece of left tibiae of 36 rabbits were resected circularly for a length of 3cm and medial cortices were partialy stripped for a length of 2.6 cm and a thickness of 1 mm.The left tibiae of the animals with unexposed marrow cavity were used as control group and the right tibiae treated in the same as the left side only with the marrow cavity drilled were used as experimental group.Six rabbits were sacrificed randomly at the end of 3rd day and 1st,2nd,4th,6th,8th weeks after operation.Tissue slice obtained from bilateral tibiae were used for immunohistochemical staining reaction of BMP and TGF βⅢ.
Results:The immunohistochemical staining reaction of BMP and TGF-βⅢ is negative in the left tibiae and there is no callus formation during the test.In the right tibiae,the staining reaction was positive at 3rd day after operation.At the end of the first week the concentration of BMP and TGF βⅢ reached the highest level and the capacity of bone formation is enhanced.Until 6 weeks the positive cells disappeared.With enhancing of bony reconstruction,the staining of bone matrix became strong.
Conclusion: BMP and TGF βⅢ are released at intervals after bone injury and they play a dominant role in bone repair. |
| KEYWORDS:Bone repair Bone morphogenetic protein Transforming growth factor β Immunohistochemistry |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 王鹏程,张英泽.内源性BMP、TGF-β在骨修复中的作用[J].中国骨伤,2000,13(7):396~397 |
| 英文格式: | WANG Peng cheng,ZHANG Ying ze.The effect of endogenous BMP and TGF-β on bone repair[J].zhongguo gu shang / China J Orthop Trauma ,2000,13(7):396~397 |
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