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补肾密骨液对骨代谢影响的体外实验研究

Hits: 2207 Download times: 1441 Received:June 11, 1998 Revised:September 28, 1998

作者	Author	单位	Unit	E-Mail
胡光亮	HU Guang liang	青岛市市立医院,山东青岛266011	Qingdao Municipal Hospital Shandong Qingdao,266011
杜靖远	DU Jing yuan	同济医科大学附属协和医院,湖北武汉
王洪	WANG Hong	同济医科大学附属协和医院,湖北武汉
谢晶		同济医科大学附属协和医院,湖北武汉

期刊信息：《中国骨伤》2000年13卷，第2期，第83-85页

DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz

目的:探讨补肾密骨液影响骨代谢的作用机制。

方法:利用组织培养技术,将不同浓度的补肾密骨液与分离培养制得的新生大鼠颅盖骨成骨细胞样细胞共同培养,用MTT比色分析法和3HTdR掺入法观察补肾密骨液对成骨细胞样细胞增殖的影响,用原子吸收分光光度法观察补肾密骨液对颅盖骨吸收的影响,并与鲑鱼降钙素进行对照。

结果:补肾密骨液以剂量依赖的方式促进成骨细胞样细胞的增殖,并抑制颅盖骨的吸收；而鲑鱼降钙素对成骨细胞样细胞的增殖无影响,但对颅盖骨的吸收有明显的抑制作用。

结论:补肾密骨液直接参与骨代谢的调节,促进骨形成,同时抑制骨吸收。

[关键词]:中草药药理学成骨细胞

Extracorporeal Experimental Study of the Effect of Bushen Migu Ye on Bony Metabolism

Abstract:

Objective:To investigate the mechanism of action of Bushen Migu Ye(BSMGY)on bony metabolism

Methods:Using tissue culture technology,the suspension of osteoblast like cells which obtained from new born mouse skullcap and BSMGY of different concentration were cultured together The effect of BSMGY on proliferation of osteoblast like cells was analysed by the colorimetric analytical method and 3H TdR incorporation method The effect of BSMGY on bone absorption was studied with atomic absorption spectropholometry Salmon calcitonin acted as comparison

Results:BSMGY directly promoted proliferation of osteoblast like cells at a dose dependent manner and inhibited bone absorption;while salmon calicitonin had no effect on proliferation of osteoblastic cells,but inhibited bone absorption obviously

Conclusion:BSMGY directly affect bony metabolism by promoting bone formation and inhibiting bone absorption

KEYWORDS:Drugs Chinese herbal Pharmacology Osteoblasts

引用本文，请按以下格式著录参考文献：

中文格式:	胡光亮,杜靖远,王洪,谢晶.补肾密骨液对骨代谢影响的体外实验研究[J].中国骨伤,2000,13(2):83~85
英文格式:	HU Guang liang,DU Jing yuan,WANG Hong.Extracorporeal Experimental Study of the Effect of Bushen Migu Ye on Bony Metabolism[J].zhongguo gu shang / China J Orthop Trauma ,2000,13(2):83~85

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