| 髓复康对脊髓半横断大鼠背根节CGRP表达的影响 |
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Received:December 30, 1998 Revised:March 10, 1999
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| 期刊信息：《中国骨伤》1999年12卷，第3期，第14-17页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:国家中医药管理局198年立项课题 |
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目的：研究中药髓复康对脊髓损伤后，背根节（DRG）神经元降钙素基因相关肽（CGRP）表达的影响。探讨髓复康效应的机理。
方法：造成下胸髓半横断损伤的Wistar大鼠模型，用免疫细胞化学法和定量图像分析法检查CGRP表达的变化。
结果：①脊髓半横断引起同侧首、尾侧各3个DRG内，CGRP表达的明显和持续性下调；②损伤后给服髓复康冲剂，可以明显减轻，但不能完全阻止，脊髓损伤诱发的CGRP表达下调；③损伤后给服中药补阳还五汤（或用大剂量的氢化考地松）治疗，不能减轻脊髓损伤诱发的CGRP表达下调；④脊髓损伤后服用髓复康的大鼠，其脊髓损伤区有明显的修复再生。
结论：结果不仅表明CGRP 表达的变化与脊髓组织的损伤和再生有一定的关联; 而且也表明, 髓复康通过对CGRP-mRNA 表达的调制, 影响神经元DNA 的合成, 可能是它促进脊髓损伤组织的修复再生的机理之一。此假设还需进一步确认。 |
| [关键词]:中药 脊髓再生 背根神经节 CGRP表达 |
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| Effect of Chinese Herb(Suifukang)on CGRP Expression in Located Dorsal Root Ganglia in Rats with Spinal Cord Hemitransection |
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Abstract:
Objective:To study effect of Suifukang on calcitonin gene related peptide (CGRP)expression in dorsal root ganglia(DRG) after spinal cord injury and to inquire into mechanism of Suifukang effect.
Methods: To make model of righthemitransection at the lower thoracic spinal cord of Wistar rats and to evaluate changes of CGRP expression with immunocytochemistry and quantitative image analysis.
Results: Spinal cord hemitransection caused a significant downregulation of CGRP expression in ipsilateral 3 DRGs oral and caudal to the injury level respectively.The downregulation of CGRP expression was consistent and irreversible during one month after spinal cord injury.The down-regulation of CGRP expression was evidently relieved but not blocked completely with administration of Suifukang.The downregulation of CGRP expression induced by spinal cord injury could not be relieved with administration of Buyanghuanwutang,or hydrocortisone in a large dose.The injured spinal tissue was repaired and regenerated in rats with administration of Suifukang.
Conclusion: The results not only suggest that there is relationship among injury,regeneration and CGRP expression in spinal cord,but also indicate that it may be one of mechanisms for promoting repair and regeneration of spinal cord injury that Suifukang modulates CGRPmRNA expression to regulate synthesis of DNA in neurons.However,the suggestions need to be further confirmed. |
| KEYWORDS:Chinese herb Spinal cord regeneration Dorsal root ganglia CGRP expression |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 韩凤岳,唐军民,刘卫东,唐岩,张平,苏衍萍,常超英,曲鹏,刘恩.髓复康对脊髓半横断大鼠背根节CGRP表达的影响[J].中国骨伤,1999,12(3):14~17 |
| 英文格式: | Han Fengyue,Tang Junmin,Liu Weidong.Effect of Chinese Herb(Suifukang)on CGRP Expression in Located Dorsal Root Ganglia in Rats with Spinal Cord Hemitransection[J].zhongguo gu shang / China J Orthop Trauma ,1999,12(3):14~17 |
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