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间断压力对离体培养成骨细胞作用的研究

Hits: 1922 Download times: 1068 Received:April 30, 1995 Revised:March 22, 1996

作者	Author	单位	Unit	E-Mail
李可心	Li Ke-xin	中国中医研究院骨伤科学究所北京 100700	Institute of Orthopaedics & Traumatology, Chinese Academy of TCM 100700
尚天裕	Shang Tian-yu	中国中医研究院骨伤科学究所北京 100700	Institute of Orthopaedics & Traumatology, Chinese Academy of TCM 100700
董福慧	Dong Fu-hui	中国中医研究院骨伤科学究所北京 100700	Institute of Orthopaedics & Traumatology, Chinese Academy of TCM 100700
张碧辉		中国科学院生物物理研究所
陈楚楚		中国科学院生物物理研究所
贝时璋		中国科学院生物物理研究所
钱民全		中国科学院力学研究所
赵笃风		中国科学院力学研究所

期刊信息：《中国骨伤》1996年9卷，第5期，第7-9页

DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz

基金项目:国家自然科学基金

实验模拟骨在功能活动过程中细胞外环境压力的变化这一生理过程,给实验组离体培养成骨细胞提供间断气体压力变化(0.098MPa,15分钟加压,15分钟无压力,2周期/小时,每天加压8小时),发现成骨细胞数逐步增加,且碱性磷酸酶活性也明显增高,均与对照组有明显差异。结果提示,间断加压可促进离体培养成骨细胞的增殖与分化。

[关键词]:间断压力成骨细胞培养

Study of the effect of intermittent compressive pressure to the osteoblasts in vitro

Abstract:The experiment imitated the physiological changes of the cellualr exteanal circumstances, existed during skeletal functional movement, and supplied a intermittent compressive pressure(0.098MPa.15 minutes pressrue, 15 minutes relax,2 cycles/one hour,8hour/day)to the osteoblasts of experiment al groups in vitro.It was discovered that the numbers of osteoblasts and the reaction of alkaline phosphatase in the experimental group were markedly elevated than that of the control groups. The results indicate that the intermittent compressive prssure is able to improve the proliferation and differentiation of the osteoblasts.

KEYWORDS:Intermittent compressive pressure Osteoblast in vitro

引用本文，请按以下格式著录参考文献：

中文格式:	李可心,尚天裕,董福慧,张碧辉,陈楚楚,贝时璋,钱民全,赵笃风.间断压力对离体培养成骨细胞作用的研究[J].中国骨伤,1996,9(5):7~9
英文格式:	Li Ke-xin,Shang Tian-yu,Dong Fu-hui.Study of the effect of intermittent compressive pressure to the osteoblasts in vitro[J].zhongguo gu shang / China J Orthop Trauma ,1996,9(5):7~9

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