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分散和植块法培养获得大量雪旺氏细胞的研究

Hits: 2088 Download times: 1264 Received:December 12, 1994 Revised:August 02, 1995

作者	Author	单位	Unit	E-Mail
李义凯	Li Yi-kai	上海中医药大学附属曙光医院	Afiliated Hospital,Shanghai University of TCM 2000211
石印玉	Shi Yin-yu	上海中医药大学附属曙光医院	Afiliated Hospital,Shanghai University of TCM 2000211
石关桐	shi Guan-tong	上海中医药大学附属曙光医院	Afiliated Hospital,Shanghai University of TCM 2000211

期刊信息：《中国骨伤》1996年9卷，第3期，第8-9页

DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz

SD乳鼠坐骨神经匀浆应用分散培养,在差速粘附贴壁的基础上,联合应用低浓度血清和抗分裂剂,可抑制和消除快速分裂的成纤维细胞,得到纯净的雪旺氏细胞。同时,取乳鼠坐骨神经和钳伤的青年大鼠坐骨神经,分别采用”反复种植法“培养,经免疫组化(抗S-100蛋白)染色证实,长出的细胞为雪旺氏细胞。

[关键词]:雪旺氏细胞坐骨神经免疫组织化学法

Study on large amount of Schwann's cell obtained in the culture of scattered andlock implantation method

Abstract:SD suckling rat sciatic nerve even paste was cultured separately, on the basis os differential adhesion stick wall, combined applica-tion of low concentration serum and anticlevage agent to inhibit and diminish quick clevage of the fibroblast in order to obtain pure Schwann's cell.Meanwhile suckling and clamp injured juvenile rat were used in repeatedly implantation culture. Through immunohistochemical stain (anti-S-100 protein) proved that the growing cell is Schwann's cell.

KEYWORDS:Schwann's cell Sciatic nerve Immunohistochemical method

引用本文，请按以下格式著录参考文献：

中文格式:	李义凯,石印玉,石关桐.分散和植块法培养获得大量雪旺氏细胞的研究[J].中国骨伤,1996,9(3):8~9
英文格式:	Li Yi-kai,Shi Yin-yu,shi Guan-tong.Study on large amount of Schwann's cell obtained in the culture of scattered andlock implantation method[J].zhongguo gu shang / China J Orthop Trauma ,1996,9(3):8~9

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