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骨关节炎发病机理的实验研究

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作者	Author	单位	Unit	E-Mail
易宪文	Yi Xian-wen	中国中医研究院骨伤科研究所	Institute of Orthopaedics and Traumatology China Academy of TCM
房世源		中国中医研究院骨伤科研究所	Institute of Orthopaedics and Traumatology China Academy of TCM
孙树椿		中国中医研究院骨伤科研究所	Institute of Orthopaedics and Traumatology China Academy of TCM

期刊信息：《中国骨伤》1990年3卷，第2期，第4-6,13页

DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz

本实验运用基质组织化学和酶组织化学的方法,通过光镜、扫描电镜和透射电镜对木瓜蛋白酯诱发的实验性骨关节炎关节软骨形态变化进行观察,发现早期变性软骨细胞中溶酶体样结构大量增多,溶酶体酶活性增强,而软骨基质糖胺多糖成份明显减少,进而产生一系列早期骨关节炎特征性损害。结果表明：糖胺多糖的减少与溶酶体酶活性升高有关。因而认为溶酶体酶活性增高是骨关节炎病变发展的机制之一。随着骨关节炎研究不断取得进展,关节软骨中酶的活性变化在这一退行性疾病中的作用,引起愈来愈多学者的重视。但在这方面生物化学文献较多,而组织化学方面的研究报道尚不多见。我们试图运用基质组织化学和酯组织化学的方法,通过光镜,扫描电镜与透射电镜对木瓜蛋白酶诱发的实验性骨关节炎的关节软骨组织化学成份变化,组织结构和超微结构的变化进行观察,以便进一步阐明骨关节炎的发病机制。

Papain induced osteoarthritis-an experimental study

Abstract:Ths is an experimental observation of osteoarthritis(OA) induced with papain injection　into the knee joints of Wistar rats. Light microscopy (LM) was done following histochemical stainings for different enzymes including LDH, SDH, G6POH, NsEase, ACPase and　AlPase.The specimens were prepared and studied under scanning and transmission electron microscopes.Remarkable OA changes were observed two weeks after the injection of papain,in comparison with the contralateral knee which was injecterd with PBS and used as control. The　effect of intra-articular injection of papain was the destruction of the cartilage matrix by redution of the proteoglycans.This is a good animal model for the study of the pathogenisis of OA.

引用本文，请按以下格式著录参考文献：

中文格式:	易宪文,房世源,孙树椿.骨关节炎发病机理的实验研究[J].中国骨伤,1990,3(2):4~6,13
英文格式:	Yi Xian-wen.Papain induced osteoarthritis-an experimental study[J].zhongguo gu shang / China J Orthop Trauma ,1990,3(2):4~6,13

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