不同糖皮质激素诱导鸡股骨头坏死的实验研究
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作者Author单位AddressE-Mail
肖春生 XIAO Chun-sheng 中国中医科学院望京医院,北京 100102 Wangjing Hospital Affiliated to China Academy of Chinese Medical Science,Beijing 100102,China  
林娜 LIN Na 中国中医科学院中药研究所 linna888@163.com 
林诗富 LIN Shi-fu 中国中医科学院望京医院,北京 100102 Wangjing Hospital Affiliated to China Academy of Chinese Medical Science,Beijing 100102,China  
万蓉 WAN Rong 中国中医科学院中药研究所  
陈卫衡 CHEN Wei-heng 中国中医科学院望京医院,北京 100102 Wangjing Hospital Affiliated to China Academy of Chinese Medical Science,Beijing 100102,China  
期刊信息:《中国骨伤》2010年,第23卷,第3期,第184-187页
DOI:10.3969/j.issn.1003-0034.2010.03.011
基金项目:国家自然科学基金面上项目(编号:30472131,30672770)
中文摘要:

目的:观察甲泼尼龙琥珀酸钠与地塞米松对鸡股骨头坏死发生的影响。

方法:健康来航鸡36只,随机分成正常组(A组)、甲泼尼龙小剂量组(B组)、甲泼尼龙大剂量组(C组)、地塞米松小剂量加马血清组(D组)、地塞米松中剂量加马血清组(E组)、地塞米松大剂量组(F组),共6组,每组6只。分别于第6、12周翼下静脉采血,检测血清总胆固醇(CHO)和甘油三酯(TG)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL);在实验12周取股骨头,行HE染色,光镜下计数空骨陷窝率,观察其病理学改变。

结果:与正常组相比,在第6周,C、F组CHO升高(P<0.05),B、C、E组TG升高(P<0.05),而B、C、E组HDL降低;在第12周,B、C、E、F组动物血TG、CHO均升高(P<0.05),C、D、E组动物血HDL降低(P<0.05),多数实验鸡血清内LDL未能检出。在第12周,C、E组股骨头空骨陷窝明显高于正常组(P<0.05),并出现较典型骨坏死病理学改变。

结论:甲泼尼龙琥珀酸钠较地塞米松更易诱导鸡早期股骨头坏死的发生,而血脂代谢紊乱可能是激素引起该股骨头坏死的重要病理基础。
【关键词】股骨头坏死    激素类  病理过程  甘油三酯类  胆固醇
 
Experimental study on avascular necrosis of femoral head in chickens induced by different Glucocorticoides
ABSTRACT  

Objective: To study the effects of Methylprednisolone and Dexamethasone on the avascular necrosis of femoral head in chickens.

Methods: Thirty-six chickens were randomly divided into 6 groups (n=6):control group (group A),Methylprednisolone low dose group(group B),Methylprednisolone large dose group(group C),small dose Dexamethasone and horse serum group(group D),middle dose Dexamethasone and horse serum group(group E),and Dexamethasone large dose group(group F). On the 6 th and 12 th weeks,blood samples were obtained to determine the level of total cholesterol triglyeride(TG),high density lipoprotein(HDL) and low density lipoprotein(LDL). On the12 th week,femoral heads were taken off. Paraffin tissue sections were prepared to detect histopathologic change with hematoxylin and eosin staining.

Results: On the 6 th week,compared with group A,the level of CHO increased significantly in group C and group F(P<0.05),and TG increased in group B,C and group E,while HDL decreased in group B,C and group E. On the 12 th week,the level of TG and CHO increased in group B,C,E and group F,and HDL decreased in group C,D and group E(P<0.05). LDL was not detected in most chickens. The ratio of empty lacuna was higher in group C and group E compared with those of the control group(P<0.05).

Conclusion: Methylprednisolone is easier to induce osteonecrosis of femoral head than Dexamethasone. The condition of metabolic disorder in blood may be the basic pathomechanism of steroid-induced necrosis of femoral head.
KEY WORDS  Femur head necrosis  Chickens  Hormones  Pathologic processes  Triglycerides  Cholesterol
 
引用本文,请按以下格式著录参考文献:
中文格式:肖春生,林娜,林诗富,万蓉,陈卫衡.不同糖皮质激素诱导鸡股骨头坏死的实验研究[J].中国骨伤,2010,23(3):184~187
英文格式:XIAO Chun-sheng,LIN Na,LIN Shi-fu,WAN Rong,CHEN Wei-heng.Experimental study on avascular necrosis of femoral head in chickens induced by different Glucocorticoides[J].zhongguo gu shang / China J Orthop Trauma ,2010,23(3):184~187
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