| 基于p38MAPK信号通路分析咪达唑仑对腰椎间盘突出症模型大鼠疼痛的影响 |
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投稿时间：2022-03-11
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| 作者 | Author | 单位 | Address | E-Mail |
| 刘建 |
LIU Jian |
开滦总医院赵各庄医院麻醉科, 河北 古治 063101 |
Department of Anesthesiology, Kailuan General Hospital Zhaogezhuang Hospital, Guzhi 063101, Hebei, China |
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| 叶玉军 |
YE Yu-jun |
唐山市中医医院麻醉科, 河北 唐山 063000 |
Department of Anesthesiology, Tangshan Hospital of Traditional Chinese Medicine, Tangshan 063000, Hebei, China |
xxhq3n@163.com |
| 刘树民 |
LIU Shu-min |
开滦总医院赵各庄医院麻醉科, 河北 古治 063101 |
Department of Anesthesiology, Kailuan General Hospital Zhaogezhuang Hospital, Guzhi 063101, Hebei, China |
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| 刘爽 |
LIU Shuang |
开滦总医院赵各庄医院外科, 河北 古治 063101 |
Department of Surgery, Kailuan General Hospital Zhaogezhuang Hospital, Guzhi 063101, Hebei, China |
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| 期刊信息：《中国骨伤》2023年，第36卷，第1期，第55-60页 |
| DOI：10.12200/j.issn.1003-0034.2023.01.010 |
| 基金项目:河北省医学科学研究计划(编号:20201290) |
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中文摘要:
目的:探究基于p38MAPK信号通路分析咪达唑仑对腰椎间盘突出症模型大鼠疼痛的影响。
方法:选取50只SPF级别SD健康大鼠,雌雄各半,随机分为正常组,模型组,低、中、高剂量组,模型组和低、中、高剂量组先建立腰椎间盘突出症模型。正常组、模型组大鼠腹腔注射生理盐水,低、中、高剂量组大鼠腹腔注射咪达唑仑,分别按30、60、90 mg/kg给药。采用酶联免疫吸检测大鼠血清中白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、5-羟色胺(5-hydroxytryptamine,5-HT)、β-内啡肽(β-endorphin,β-EP)、P物质(substance P,SP)、神经肽Y(neuropeptide Y,NPY)水平,采用Western blot检测各组大鼠组织中p38 MAPK,基质金属蛋白酶3(matrix metalloproteinase-3,MMP3)蛋白表达。
结果:模型组大鼠TNF-α、IL-1β、β-EP水平较正常组高,5-HT水平低于正常组(P＜0.05);低、中、高剂量组大鼠TNF-α、IL-1β、β-EP水平较模型组下降、5-HT水平升高(P＜0.05)。模型组大鼠较正常组SP、NPY水平上升(P＜0.05);低、中、高剂量组较模型组大鼠SP、NPY水平下降(P＜0.05)。模型组较正常组大鼠p38 MAPK、MMP-3表达上升(P＜0.05),低、中、高剂量较模型组大鼠p38 MAPK、MMP-3表达下降(P＜0.05)。
结论:咪达唑仑可以改善腰椎间盘突出症模型大鼠的免疫炎症反应,可能是通过p38MAPK信号通路调控来实现的。 |
| 【关键词】p38MAPK信号通路 椎间盘移位 免疫 炎症 腰椎 疼痛 |
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| Analysis of the effect of midazolam on pain in a rat model of lumbar disc herniation based on the p38 MAPK signaling pathway |
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ABSTRACT
Objective To investigate the effect of midazolam on pain in lumbar disc herniation model rats based on p38 MAPK signaling pathway.
Methods Fifty SPF-grade Sprague-Dawley healthy rats,half male and half female,were selected and randomly divided into normal group,model group,and low-dose,medium-dose,high-dose groups. Model group and low-dose,medium-dose,high-dose groups were initially modeled for lumbar disc herniation. Intraperitoneal injection of saline was performed in rats of normal and model groups; and in the low-dose,medium-dose,and high-dose groups,intraperitoneal injection of midazolam was performed with doses of 30,60,and 90 mg/kg,respectively. Interleukin-1β (IL-1β),tumor necrosis factor-α (TNF-α),5-hydroxytryptamine (5-HT),β-endorphin (β-EP),substance P (SP),neuropeptide Y (NPY) were detected in the serum of rats by enzyme-linked immunoassay. The expression of p38 MAPK and matrix metalloproteinase-3(MMP-3) protein were detected by Western blot in the tissues of rats of each group.
Results The levels of TNF-α,IL-1β and β-EP were higher and the level of 5-HT was lower in the model group than in the normal group(P＜0.05);the levels of TNF-α,IL-1β and β-EP were lower and the level of 5-HT was higher in the low-dose,medium-dose and high-dose groups than in the model group(P＜0.05). The levels of SP and NPY increased in the model group compared with the normal group (P＜0.05) and the levels of SP and NPY decreased in the low-dose,medium-dose and high-dose groups compared with the model group (P＜0.05). The expression of p38 MAPK and MMP-3 increased in the model group compared with the normal group (P＜0.05); the expression of p38 MAPK and MMP-3 decreased in the low-dose,medium-dose and high-dose compared with the model group(P＜0.05).
Conclusion Midazolam may ameliorate the immune inflammatory response in rats with a model of lumbar disc herniation,possibly regulated through the p38MAPK signaling pathway. |
| KEY WORDS p38MAPK signaling pathway Intervertebral disk displacement Immunity Inflammation Lumbar vertebrae Pain |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 刘建,叶玉军,刘树民,刘爽.基于p38MAPK信号通路分析咪达唑仑对腰椎间盘突出症模型大鼠疼痛的影响[J].中国骨伤,2023,36(1):55~60 |
| 英文格式: | LIU Jian,YE Yu-jun,LIU Shu-min,LIU Shuang.Analysis of the effect of midazolam on pain in a rat model of lumbar disc herniation based on the p38 MAPK signaling pathway[J].zhongguo gu shang / China J Orthop Trauma ,2023,36(1):55~60 |
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