亚麻木酚素对大鼠生长板软骨细胞凋亡的影响
摘要点击次数: 43   全文下载次数: 16   投稿时间:2022-05-20    
作者Author单位AddressE-Mail
梁国辉 LIANG Guo-hui 洛阳正骨医院 河南省骨科医院, 河南 洛阳 471000 Henan Provincial Luoyang Orthopedic-Traumatological Hospital, Henan Provincial Orthopedic Hospital, Luoyang 471000, Henan, China  
谢艳 XIE Yan 洛阳正骨医院 河南省骨科医院, 河南 洛阳 471000 Henan Provincial Luoyang Orthopedic-Traumatological Hospital, Henan Provincial Orthopedic Hospital, Luoyang 471000, Henan, China xieyan1666@126.com 
郭云鹏 GUO Yun-peng 洛阳正骨医院 河南省骨科医院, 河南 洛阳 471000 Henan Provincial Luoyang Orthopedic-Traumatological Hospital, Henan Provincial Orthopedic Hospital, Luoyang 471000, Henan, China  
邢伟鹏 XING Wei-peng 洛阳正骨医院 河南省骨科医院, 河南 洛阳 471000 Henan Provincial Luoyang Orthopedic-Traumatological Hospital, Henan Provincial Orthopedic Hospital, Luoyang 471000, Henan, China  
裴圆圆 PEI Yuan-yuan 洛阳正骨医院 河南省骨科医院, 河南 洛阳 471000 Henan Provincial Luoyang Orthopedic-Traumatological Hospital, Henan Provincial Orthopedic Hospital, Luoyang 471000, Henan, China  
期刊信息:《中国骨伤》2022年,第35卷,第11期,第1087-1094页
DOI:10.12200/j.issn.1003-0034.2022.11.015
基金项目:河南省中医药科学研究专项课题(编号:2018ZY1023)
中文摘要:

目的: 比较不同浓度亚麻木酚素抑制生长板软骨细胞凋亡的效果,筛选出最适药物浓度,为其延迟大鼠骨骺闭合促进长骨生长进行理论支持。

方法: 选取2只4周龄雄性SD大鼠,SPF级,体质量80 g。解剖大鼠胫骨及股骨生长板软骨并进行体外分离,获得生长板软骨细胞进行培养,利用倒置相差显微镜及Ⅱ型胶原免疫荧光实验对软骨细胞分别进行形态学观察、鉴定,然后采用白细胞介素-1β(interleukin-1β,IL-1β)20 ng/ml诱发生长板软骨细胞凋亡为模型组,同时加入分别含1、10、20、40 μM亚麻木酚素为实验组,同时加入5 μM来曲唑为阳性对照组,分别培养24、48 h,MTT法观察药物促进细胞增殖情况,流式细胞仪检测药物抑制细胞凋亡情况。

结果: 含量1、10、20、40 μM的亚麻木酚素均可不同程度促进细胞增殖,其原理为药物抑制了IL-1β诱发的生长板软骨细胞凋亡,抑制细胞凋亡的最佳药物浓度为20 μM。

结论: 适宜浓度的亚麻木酚素对大鼠生长板软骨细胞凋亡抑制效果明显且最适药物浓度为20 μM,为骨骼延迟闭合提供更长的生长时间,从而为促进发育期骨骼增长提供可能。
【关键词】亚麻木酚素  软骨细胞  细胞凋亡  细胞抑制  骨骺闭合
 
Effect of Flax lignans on apoptosis of growth plate chondrocytes in rats
ABSTRACT  

Objective: To compare the effects of different concentrations of linolenin on inhibiting apoptosis of chondrocytes in the growth plate,and to screen the optimal concentration of linolenin,so as to provide theoretical support for delaying epiphyseal closure and promoting long bone growth in rats.

Methods: Two 4-week-old male SD rats (SPF grade) with a body mass of 80 g were selected. The growth plate cartilage of rat tibia and femur was dissected and isolated in vitro to obtain growth plate chondrocytes for culture. The chondrocytes were observed and identified by inverted phase contrast microscope and typeⅡ collagen immunofluorescence test,and then 20 ng/ml IL-1β was used to induce apoptosis of growth plate chondrocytes as model group,and added with 1,10,20,40 μM linolenin as the experimental group,and 5 μM letrozole as the positive control group. The cells were cultured for 24 and 48 hours respectively. The drug promoted cell proliferation was observed by MTT method,and the drug inhibited cell apoptosis was detected by flow cytometry.

Results: Contents 1,10,20,40 μM could promote cell proliferation in varying degrees,and the principle was that the drug inhibits IL-1β induced chondrocyte apoptosis in the growth plate,and the optimal concentration of drugs to inhibit apoptosis was 20 μM.

Conclusion: The appropriate concentration of linseed lignans can significantly inhibit the apoptosis of chondrocytes in the growth plate of rats,and the optimal drug concentration is 20 μM. It provides possibility for delayed bone closure and longer growth time to promote bone growth during development.
KEY WORDS  Flax lignans  Chondrocytes  Apoptosis  Cell inhibition  Epiphyseal closure
 
引用本文,请按以下格式著录参考文献:
中文格式:梁国辉,谢艳,郭云鹏,邢伟鹏,裴圆圆.亚麻木酚素对大鼠生长板软骨细胞凋亡的影响[J].中国骨伤,2022,35(11):1087~1094
英文格式:LIANG Guo-hui,XIE Yan,GUO Yun-peng,XING Wei-peng,PEI Yuan-yuan.Effect of Flax lignans on apoptosis of growth plate chondrocytes in rats[J].zhongguo gu shang / China J Orthop Trauma ,2022,35(11):1087~1094
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