| 基于β-catenin信号通路内热针治疗腰椎退行性病变机制研究 |
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投稿时间：2020-03-15
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| 作者 | Author | 单位 | Address | E-Mail |
| 庄金刚 |
ZHUANG Jin-gang |
北京中医药大学深圳医院, 广东 深圳 518000 |
Shenzhen Hospital of Beijing University of Traditional Chinese Medicine, Shenzhen 518000, Guangdong, China |
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| 张雄波 |
ZHANG Xiong-bo |
北京中医药大学深圳医院, 广东 深圳 518000 |
Shenzhen Hospital of Beijing University of Traditional Chinese Medicine, Shenzhen 518000, Guangdong, China |
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| 陈冬 |
CHEN Dong |
北京中医药大学深圳医院, 广东 深圳 518000 |
Shenzhen Hospital of Beijing University of Traditional Chinese Medicine, Shenzhen 518000, Guangdong, China |
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| 程肖芳 |
CHENG Xiao-fang |
北京中医药大学深圳医院, 广东 深圳 518000 |
Shenzhen Hospital of Beijing University of Traditional Chinese Medicine, Shenzhen 518000, Guangdong, China |
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| 温建民 |
WEN Jian-min |
中国中医科学院望京医院, 北京 100102 |
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| 王圆圆 |
and WANG Yuan-yuan |
北京中医药大学深圳医院, 广东 深圳 518000 |
Shenzhen Hospital of Beijing University of Traditional Chinese Medicine, Shenzhen 518000, Guangdong, China |
wangyy325@163.com |
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| 期刊信息：《中国骨伤》2020年，第33卷，第5期，第484-488页 |
| DOI：10.12200/j.issn.1003-0034.2020.05.019 |
| 基金项目:深圳市龙岗区科技发展专项资金（编号：LGKCYLWS2018000048）；深圳市医疗卫生三名工程项目（编号：SZSM201805089） |
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中文摘要:
目的：通过观察内热针对腰椎退行性病变大鼠椎间盘组织分泌型糖蛋白（Wnt1）、轴蛋白（Axin）及β-链蛋白（β-catenin）表达的影响，探讨内热针对腰椎退行性病变大鼠纤维环细胞凋亡的调节作用机制。
方法：采用Sun等造模方法，将40只SD大鼠随机分为正常组、模型组、内热针组、针刺组，每组10只，除了正常组大鼠外，余均进行造模。选取双侧大肠俞进行内热针及针刺治疗。采用Western-bolt法检测大鼠椎间盘组织Wnt1、Axin及β-catenin的表达。
结果：与模型组相比，内热针组和针刺组Wnt1和β-catenin表达均显著下降（P<0.05）；内热针组大鼠纤维环Axin表达显著增加（P<0.05），针刺组Axin表达差异无统计学意义（P>0.05）。
结论：内热针能下调腰椎退行性病变大鼠纤维环中Wnt1和β-catenin的表达，上调Axin的表达，这表示该机制可能通过调节β-catenin信号通路相关因子的表达水平来治疗腰椎退行性疾病。 |
| 【关键词】β-catenin信号通路 内热针 腰椎退行性病变 |
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| Inner-heating acupuncture of lumbar degenerative lesions based on β-catenin signaling pathway |
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ABSTRACT
Objective: By observing the effect of inner-heating acupuncture on the expression of Wnt1,Axin and β-catenin in the intervertebral disc of rats with lumbar degenerative disease and to explore the regulatory mechanism of inner-heating acupuncture on the apoptosis of annulus fibrosus cells in rats with lumbar degenerative disease.
Methods: Forty SD rats were randomly divided into normal group,model group,inner-heating acupuncture group and acupuncture group with 10 rats in each group. Except for normal group,all rats were modeled. Bilateral Da Chang Shu were selected for inner-heating acupuncture and acupuncture treatment. Western-bolt assay was used to detect the expression of Wnt1,Axin and β-catenin in rat intervertebral discs.
Results: Compared with the model group,Wnt1 and β-catenin expression in annulus fibrosus cells of rats in the inner-heating acupuncture group and the acupuncture group were significantly decreased(P<0.05);the expression of Axin in annulus fibrosus of rats in the inner-heating acupuncture group was significantly increased(P<0.05). There was no significant difference in the expression of Axin in annulus fibrosus of rats between acupuncture group and model group(P>0.05).
Conclusion: Inner-heating acupuncture can down-regulate the expression of Wnt1 and β-catenin and up-regulate the expression of Axin in annulus fibrosus of rats with lumbar degenerative desease. It is suggested that the mechanism may be to regulate the expression of related factors in the β-catenin signaling pathway,so as to achieve the goal of treating lumbar degenerative diseases. |
| KEY WORDS β-catenin signaling pathway Inner-heating acupuncture Lumbar degenerative diseases |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 庄金刚,张雄波,陈冬,程肖芳,温建民,王圆圆.基于β-catenin信号通路内热针治疗腰椎退行性病变机制研究[J].中国骨伤,2020,33(5):484~488 |
| 英文格式: | ZHUANG Jin-gang,ZHANG Xiong-bo,CHEN Dong,CHENG Xiao-fang,WEN Jian-min,and WANG Yuan-yuan.Inner-heating acupuncture of lumbar degenerative lesions based on β-catenin signaling pathway[J].zhongguo gu shang / China J Orthop Trauma ,2020,33(5):484~488 |
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