| 消肿止痛膏对腓肠肌损伤大鼠MEF2 mRNA与蛋白表达的影响 |
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投稿时间：2018-09-20
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| 作者 | Author | 单位 | Address | E-Mail |
| 王钢 |
WANG Gang |
中国中医科学院望京医院骨伤综合科, 北京 100102 |
Department of Orthopaedics and Trauma, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China |
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| 赵勇 |
ZHAO Yong |
中国中医科学院望京医院骨伤综合科, 北京 100102 |
Department of Orthopaedics and Trauma, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China |
zhaoyong423@163.com |
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| 期刊信息：《中国骨伤》2019年，第32卷，第6期，第578-581页 |
| DOI：10.3969/j.issn.1003-0034.2019.06.019 |
| 基金项目:2012年北京市科委中医药外治法科技项目（编号：WZF2012-01） |
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中文摘要:
目的：基于微小RNA表达机制，探讨消肿止痛膏对大鼠骨骼肌损伤后重塑和修复的影响。
方法：通过钝挫伤模型的方法建立大鼠腓肠肌损伤模型，观察消肿止痛膏对大鼠损伤后4、7、14、21 d大鼠定量PCR检测腓肠肌MEF2 mRNA与蛋白质的表达水平，探讨消肿止痛膏对大鼠腓肠肌钝挫伤模型的肌肉重塑与修复的作用机制。
结果：治疗组MEF2的表达水平高于空白组与模型组，进一步证实了MEF2在外用药物中引起骨骼肌重塑及修复过程中的重要作用。MEF2在损伤后7 d上升，其后表达一直维持在较高水平，至损伤后21 d仍可以表达出较高水平。与模型组相比较，其表达的高峰期在14 d左右，其后则基本回归到一般状态。
结论：MEF2的表达水平呈现上升趋势，即使至损伤后21 d，其表达仍未见明显下降，可见消肿止痛软膏可以促进MEF2的表达。同时，消肿止痛膏可以调节骨骼肌的再生修复，因此，消肿止痛膏可以通过基因调控，发挥对骨骼肌损伤后的再生修复作用。 |
| 【关键词】腓肠肌 创伤和损伤 生肌调节因子 植物药疗法 大鼠 |
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| Effect of Xiaozhong Zhitong Ointment(消肿止痛膏)on MEF2 mRNA and protein expression in rats with gastrointestinal muscle injury |
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ABSTRACT
Objective:To investigate the effect of Xiaozhong Zhitong Ointment(消肿止痛膏,XZZTO)on remodeling and repair of skeletal muscle injury in rats based on the expression mechanism of microRNA.
Methods:The rat gastrocnemius injury model was established by blunt contusion model. The expression of MEF2 gene and protein in gastrocnemius muscle was detected by quantitative PCR at 4,7,14 and 21 days after injury with XZZTO. The mechanism of the effect of XZZTO on the muscle remodeling and repair of rat gastrocnemius contusion model was discussed.
Results:The expression level of MEF2 in the treatment group was significantly higher than that of the control group and model group,which further confirmed the important role of MEF2 in inducing skeletal muscle remodeling and repair process in the topical drugs. The expression of MEF2 increased at 7 days after injury and remained at a high level until 21 days after injury. Compared with the model group,the peak expression period was about 14 days,and then returned to the general state.
Conclusion:The expression level of MEF2 shows an upward trend. Even 21 days after injury,the expression of MEF2 dose not show a significant downward trend. It can be seen that XZZTO can promote the expression of MEF2. At the same time,XZZTO can regulate the regeneration and repair of skeletal muscle. Therefore,XZZTO can play a regeneration and repair role after skeletal muscle injury through gene regulation. |
| KEY WORDS Muscle skeletal Wounds and injuries Myogenic regulatory factor Phytotherapy Rats |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 王钢,赵勇.消肿止痛膏对腓肠肌损伤大鼠MEF2 mRNA与蛋白表达的影响[J].中国骨伤,2019,32(6):578~581 |
| 英文格式: | WANG Gang,ZHAO Yong.Effect of Xiaozhong Zhitong Ointment(消肿止痛膏)on MEF2 mRNA and protein expression in rats with gastrointestinal muscle injury[J].zhongguo gu shang / China J Orthop Trauma ,2019,32(6):578~581 |
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