| JAK2/STAT3信号通路介导姜黄素在骨性关节炎软骨细胞代谢中的影响 |
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投稿时间：2015-12-31
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| 作者 | Author | 单位 | Address | E-Mail |
| 李旭升 |
LI Xu-sheng |
兰州军区总医院全军骨科中心关节外科, 甘肃 兰州 730050 |
Department of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, Gansu, China |
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| 陈慧 |
CHEN Hui |
兰州军区总医院全军骨科中心关节外科, 甘肃 兰州 730050 |
Department of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, Gansu, China |
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| 甄平 |
ZHEN Ping |
兰州军区总医院全军骨科中心关节外科, 甘肃 兰州 730050 |
Department of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, Gansu, China |
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| 李慎松 |
LI Shen-song |
兰州军区总医院全军骨科中心关节外科, 甘肃 兰州 730050 |
Department of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, Gansu, China |
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| 周胜虎 |
ZHOU Sheng-hu |
兰州军区总医院全军骨科中心关节外科, 甘肃 兰州 730050 |
Department of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, Gansu, China |
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| 田琦 |
TIAN Qi |
兰州军区总医院全军骨科中心关节外科, 甘肃 兰州 730050 |
Department of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, Gansu, China |
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| 石杰 |
SHI Jie |
甘肃省中医药大学, 甘肃 兰州 730000 |
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| 何晓乐 |
HE Xiao-le |
第四军医大学西京医院老年病科, 陕西 西安 710032 |
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| 刘军 |
LIU Ju |
兰州军区总医院全军骨科中心关节外科, 甘肃 兰州 730050 |
Department of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, Gansu, China |
tutuhehtt@126.com |
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| 期刊信息：《中国骨伤》2016年，第29卷，第12期，第1104-1109页 |
| DOI：10.3969/j.issn.1003-0034.2016.12.008 |
| 基金项目:国家自然科学基金资助项目（编号：81371983）；甘肃省科技支撑计划（编号：S04671）；甘肃省自然科学基金项目（编号：1606RJZA208）；甘肃省青年科技基金项目（编号：1606RJYA300） |
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中文摘要:
目的：探讨酪氨酸蛋白激酶2/信号转导和转录激活子3（JAK2/STAT3）信号通路对软骨细胞代谢的影响以及线粒体抗氧化应激能力的改变，及JAK2/STAT3信号通路在此过程中的作用以及姜黄素对其影响。
方法：选取雄性SPF级C57BL/6小鼠15只，体重10.05~15.00 g，平均12.80 g，随机分为对照组、OA组（Glasson法建立OA模型）及姜黄素+OA组（在OA组处理的基础上，术后每日腹腔注射100 mg/kg姜黄素），每组各5只。4周后用脱颈法处死3组小鼠取材，观察各组大体标本形态学变化及采用免疫组织化学法观察标本改变；采用Western-blot蛋白印迹法检测各组p-JAK2、p-STAT3和Bax蛋白的表达，同时检测各组线粒体氧化应激指标琥珀酸脱氢酶（SDH）及细胞色素C氧化酶（COX）改变。
结果：4周后，在软骨组织标本形态学方面，对照组软骨组织呈半透明状，无肿胀、充血，软骨细胞数量多，胞核呈椭圆形，染色质分布均匀；姜黄素+OA组关节轻微肿胀，无充血，软骨细胞形态较规则，细胞数量略减少；OA组关节面粗糙，表面变薄、有轻度破损现象，细胞排列稍紊乱，视野下可见核消失，染色不均匀。与对照组相比，OA组和姜黄素+OA组软骨细胞p-JAK2、p-STAT3蛋白表达降低（P<0.05）、Bax蛋白表达升高（P<0.05），SDH和COX蛋白水平表达均降低（P<0.05）；与OA组比较，姜黄素+OA组软骨细胞p-JAK2、p-STAT3蛋白表达升高，Bax蛋白表达降低，SDH和COX蛋白水平表达均升高，差异有统计学意义（P<0.05）。
结论：JAK2/STAT3信号通路与骨性关节炎软骨退变的病理过程密切相关，姜黄素可激活JAK2/STAT3信号通路，增加软骨细胞线粒体抗氧化应激能力，明显缓解关节软骨的退变，降低OA进展水平。 |
| 【关键词】骨关节炎 姜黄素 软骨细胞 代谢 |
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| JAK2/STAT3 signal pathway mediating curcumin in cartilage cell metabolism of osteoarthritis |
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ABSTRACT
Objective: To observe influence of JAK2/STAT3 signal pathway mediating curcumin in cartilage cell metabolism of osteoarthritis and mitochondria oxidative stress resistance;also explore the role of JAK2/STAT3 signal pathway and effect of curcumin in this process.
Methods: Fifteen male SPF C57BL/6 rats rweighted from 10.05 to 15.00 g with an average of 12.80 g were collected and randomly divided into control group,OA group (modeled as OA by Glasson SS),curcumin with OA group (100 mg/kg curcumin were performed intraperitoneal injection every day based on OA group),5 rats in each group. Rats were taken off the neck after 4 weeks, morphologic change were observed,specimens changes were observed by histochemical methods;p-JAK2,p-STAT3 and Bax protein expression were detected by Western blot;At the same time,the changes of mitochondria oxidative stress index such as succinate dehydrogenase (SDH) and cytochrome C oxydase (COX) in each group were detected.
Results: After 4 weeks,cartilage tissue showed translucent shape,no swelling and congestion,the number of cartilage cell increased,nuclei liked oval,chromatin arranged uniform in control group;in curcumin with OA group,joint showed slightly swelling without congestion,the formation of cartilage cell was regular,and number of cell decreased;while in OA group,the surface of joint was roughness with mild damage,cell arrangement was a bit disorder,nuclear was disappeared under vision and dyeing was uneven. Compared with control group,p-JAK2,p-STAT3 protein expression was decreased in OA and curcumin with OA group (P<0.05),Bax protein expression was increased (P<0.05),SDA and COX protein expression were reduced (P<0.05). Compared with OA group,p-JAK2,p-STAT3 protein expression was increased in curcumin with OA,Bax protein expression was decreased (P<0.05),SDA and COX protein expression were increased (P<0.05),and had statistical differences among three groups.
Conclusion: JAK2/STAT3 signal pathway is closely associated with pathology course of osteoarthritis,curcumin could stimulate JAK2/STAT3 signal pathway and promote mitochondria oxidative stress. It could obviously relieve degeneration of articular cartilage and slow lower progress of OA. |
| KEY WORDS Osteoarthritis Curcumin Chondrocytes Metabolism |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 李旭升,陈慧,甄平,李慎松,周胜虎,田琦,石杰,何晓乐,刘军.JAK2/STAT3信号通路介导姜黄素在骨性关节炎软骨细胞代谢中的影响[J].中国骨伤,2016,29(12):1104~1109 |
| 英文格式: | LI Xu-sheng,CHEN Hui,ZHEN Ping,LI Shen-song,ZHOU Sheng-hu,TIAN Qi,SHI Jie,HE Xiao-le,LIU Ju.JAK2/STAT3 signal pathway mediating curcumin in cartilage cell metabolism of osteoarthritis[J].zhongguo gu shang / China J Orthop Trauma ,2016,29(12):1104~1109 |
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