| 脂蛋白基因在早期骨关节炎软骨下骨的表达 |
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摘要点击次数: 2073
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投稿时间：2013-07-09
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| 期刊信息：《中国骨伤》2014年，第27卷，第1期，第54-57页 |
| DOI：10.3969/j.issn.1003-0034.2014.01.014 |
| 基金项目:国家863高技术研究发展计划资助项目（编号：2008AA02Z437） |
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中文摘要:
目的：研究早期骨关节炎软骨下骨脂蛋白相关的基因表达改变情况。
方法：大鼠分为实验组（15只）和对照组（15只）.实验组切除右膝内侧半月板及内侧副韧带，对照组仅切开关节囊。于术后1、2、4周取右膝关节标本，采用全基因表达谱芯片技术研究软骨下骨全基因表达，利用差异基因分析的方法分析脂蛋白相关的基因表达改变情况。
结果：软骨下骨Apoa5表达于建模术后1周上调，术后2周下调；Apoc2表达于术后2周上调；Apol3表达于术后1周上调，术后4周下调；Lrp1于术后1、2周下调；Lrp5于术后2周下调；Gpihbp1、Lpl、Tfpi、Vldlr表达于术后1周均上调；Lrpap1、RGD1309808于术后4周均下调。
结论：脂蛋白相关基因的表达改变在早期膝关节骨关节炎的软骨下骨变化中可能起了重要作用。 |
| 【关键词】基因表达 芯片分析技术 骨关节炎,膝 软骨 脂蛋白类 |
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| Expression of lipoprotein related genes in subchondral bone of early experimental osteoarthritis |
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ABSTRACT
Objective: To study the expression of lipoprotein related genes in subchondral bone of early experimental osteoarthritis,which may play an important role in the pathogenesis of osteoarthritis.
Methods: Animals are equally divided into two groups:experimental group and control group,both of which contain fifteen rats of similar weight. The right knee joints of experimental group underwent surgery,which involved in both medial collateral ligament（MCL） transaction and medial meniscectomy,while the control group was only carried out with a sham operation. Rats were killed at 1,2 and 4 weeks postsurgery to obtain the right knee joints. Total RNA of the subchondral bone was extracted,and then hybridized to Agilent Whole Rat Genome Microarray. Differentially expressed genes analysis was used to study the chemokine signaling pathway.
Results: Apoa5 expression was down-regulated at 1,2 weeks post-surgery,Apoc2 expression was up-regulated at 1 week after surgery,Apol3 expression was up-regulated at 1 and down-regulated at 4 weeks post-surgery,Lrp1 expression was down-regulated at 1,2 weeks after surgery. Lrp5 was down-regulated at 2 weeks after surgery. Gpihbp1,Lpl,Tfpi and Vldlr were up-regulated at 1 weeks after surgery. Lrpap1 and RGD1309808 were down-regulated at 4 weeks after surgery.
Conclusion: Dysregulation of lipoprotein related genes plays an important role in pathogenesis of early experimental osteoarthritis. |
| KEY WORDS Gene expression Microchip analytical procedures Osteoarthritis,knee Cartilage Lipoproteins |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 张荣凯,杨禄坤,黄丽娟,赵庆,蔡道章.脂蛋白基因在早期骨关节炎软骨下骨的表达[J].中国骨伤,2014,27(1):54~57 |
| 英文格式: | ZHANG Rong-kai,YANG Lu-kun,HUANG Li-juan,ZHAO Qing,CAI Dao-zhang.Expression of lipoprotein related genes in subchondral bone of early experimental osteoarthritis[J].zhongguo gu shang / China J Orthop Trauma ,2014,27(1):54~57 |
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