| 京尼平苷对硝普钠诱导软骨细胞凋亡与细胞周期的影响 |
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投稿时间：2012-09-20
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| 作者 | Author | 单位 | Address | E-Mail |
| 陈万军 |
CHEN Wan-jun |
三峡大学第一临床医学院 宜昌市中心人民医院骨科, 湖北 宜昌 443002 |
Department of Orthopaedics, the First College of Clinical Medical Science, China Three Gorges University, Yichang Central People's Hospital, Yichang 443002, Hubei, China |
baotongzhu1963@163.com |
| 鲍同柱 |
BAO Tong-zhu |
三峡大学第一临床医学院 宜昌市中心人民医院骨科, 湖北 宜昌 443002 |
Department of Orthopaedics, the First College of Clinical Medical Science, China Three Gorges University, Yichang Central People's Hospital, Yichang 443002, Hubei, China |
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| 陈恳 |
CHEN Ken |
三峡大学第一临床医学院 宜昌市中心人民医院骨科, 湖北 宜昌 443002 |
Department of Orthopaedics, the First College of Clinical Medical Science, China Three Gorges University, Yichang Central People's Hospital, Yichang 443002, Hubei, China |
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| 朱长谋 |
ZHU Chang-mou |
三峡大学第一临床医学院 宜昌市中心人民医院骨科, 湖北 宜昌 443002 |
Department of Orthopaedics, the First College of Clinical Medical Science, China Three Gorges University, Yichang Central People's Hospital, Yichang 443002, Hubei, China |
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| 万峰 |
WAN Feng |
三峡大学第一临床医学院 宜昌市中心人民医院骨科, 湖北 宜昌 443002 |
Department of Orthopaedics, the First College of Clinical Medical Science, China Three Gorges University, Yichang Central People's Hospital, Yichang 443002, Hubei, China |
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| 谭玉林 |
TAN Yu-lin |
三峡大学第一临床医学院 宜昌市中心人民医院骨科, 湖北 宜昌 443002 |
Department of Orthopaedics, the First College of Clinical Medical Science, China Three Gorges University, Yichang Central People's Hospital, Yichang 443002, Hubei, China |
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| 鄢飞 |
YAN Fei |
三峡大学第一临床医学院 宜昌市中心人民医院骨科, 湖北 宜昌 443002 |
Department of Orthopaedics, the First College of Clinical Medical Science, China Three Gorges University, Yichang Central People's Hospital, Yichang 443002, Hubei, China |
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| 期刊信息：《中国骨伤》2013年，第26卷，第3期，第232-235页 |
| DOI：10.3969/j.issn.1003-0034.2013.03.015 |
| 基金项目:湖北省自然科学基金资助项目(编号;2010CDB10701) |
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中文摘要:
目的：研究京尼平苷(Geniposide)对硝普钠(SNP)诱导软骨细胞凋亡与细胞周期的影响。
方法：3周龄SD大鼠,用于分离培养软骨细胞。将第Ⅱ代软骨细胞进行分组,噻唑兰染色(MTT)法检测细胞增殖,流式细胞仪检测细胞周期及凋亡率,硝酸还原酶法检测培养液中NO含量。
结果：京尼平苷干预SNP诱导软骨细胞凋亡后,细胞处于G0/G1期的百分比减少,S及G2/M期的细胞百分比增加;同时显着降低软骨细胞凋亡率及培养液中NO含量(r=0.917,P<0.01).
结论：京尼平苷干预SNP诱导软骨细胞凋亡,可降低培养液中NO含量,促进细胞增殖,这可能是京尼平苷治疗骨性关节炎的作用机制之一。 |
| 【关键词】骨关节炎 软骨细胞 细胞,培养的 细胞凋亡 一氧化氮 |
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| Effects of Geniposide on SNP-induced apoptosis of chondrocyte and cell cycle |
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ABSTRACT
Objective: To study the effects of Geniposide on SNP(sodium nitroprusside)-induced apoptosis of chondrocyte in vitro and cell cycle.
Methods: The chondrocyte of three-week-old SD rats were separated and cultivated. The second generation of chondrocyte cells were involved in experiment. Chondrocyte proliferation was measured by MTT assay;flow cytometer were adopted to observe cell cycle and apoptosis rate;NO examination adopted nitrate reductase method.
Results: Geniposide could significantly decrease the percentage of SNP-induced chondrocytes in G0/G1 phase and increased percentage in S phase and G2/M phase. The apoptosis of chondrocyte and the concentration of NO in the culture supernatants was reduced significantly(r=0.917,P<0.01).
Conclusion: Geniposide could impact SNP-induced apoptosis of chondrocyte by reducing the concentration of NO in the culture supernatants,promoting proliferation of chondrocytes,which is a probable and important mechanism of Geniposide preventing osteoarthritis. |
| KEY WORDS Osteoarthritis Chondrocytes Cells,cultured Apoptosis Nitric oxide |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 陈万军,鲍同柱,陈恳,朱长谋,万峰,谭玉林,鄢飞.京尼平苷对硝普钠诱导软骨细胞凋亡与细胞周期的影响[J].中国骨伤,2013,26(3):232~235 |
| 英文格式: | CHEN Wan-jun,BAO Tong-zhu,CHEN Ken,ZHU Chang-mou,WAN Feng,TAN Yu-lin,YAN Fei.Effects of Geniposide on SNP-induced apoptosis of chondrocyte and cell cycle[J].zhongguo gu shang / China J Orthop Trauma ,2013,26(3):232~235 |
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