| 针刀松解法对膝骨关节炎大鼠中枢不同部位亮氨酸-脑啡肽的影响 |
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投稿时间：2010-09-03
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| 作者 | Author | 单位 | Address | E-Mail |
| 郭长青 |
GUO Chang-qing |
北京中医药大学针灸推拿学院,北京 100029 |
College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China |
guochangqing66@163.com |
| 嵇波 |
JI Bo |
北京中医药大学针灸推拿学院,北京 100029 |
College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China |
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| 陈幼楠 |
CHEN You-nan |
北京中医药大学针灸推拿学院,北京 100029 |
College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China |
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| 钟鼎文 |
ZHONG Ding-wen |
北京中医药大学针灸推拿学院,北京 100029 |
College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China |
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| 金燕 |
JIN Yan |
北京中医药大学针灸推拿学院,北京 100029 |
College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China |
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| 刘清国 |
LIU Qing-guo |
北京中医药大学针灸推拿学院,北京 100029 |
College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China |
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| 郭孟玮 |
GUO Meng-wei |
北京中医药大学针灸推拿学院,北京 100029 |
College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China |
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| 张义 |
ZHANG Yi |
北京中医药大学针灸推拿学院,北京 100029 |
College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China |
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| 刘乃刚 |
LIU Nai-gang |
北京中医药大学针灸推拿学院,北京 100029 |
College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China |
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| 陈占禄 |
CHEN Zhan-lu |
北京中医药大学针灸推拿学院,北京 100029 |
College of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029,China |
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| 期刊信息：《中国骨伤》2011年，第24卷，第8期，第656-658页 |
| DOI：10.3969/j.issn.1003-0034.2011.08.010 |
| 基金项目:国家重点基础研究发展计划(973计划)(编号:2006CB504508) |
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中文摘要:
目的: 通过观察针刀松解法干预后膝骨关节炎(KOA)大鼠中枢不同部位亮氨酸-脑啡肽(L-ENK)的变化并与电针干预相比较,探讨针刀松解法在脊髓及脊髓以上水平的镇痛机制.
方法: 将60只健康SD大鼠随机分为空白组、模型组、电针组和针刀组.将4%木瓜蛋白酶溶液与0.3 mol/L半胱氨酸溶液按1∶1混合静置0.5 h后分别于造模第1、4、7天注射于后3组大鼠左膝关节腔,每次20 μl.造模第36天开始,相应给予针刀松解治疗或电针治疗,治疗3周后取大鼠腰膨大处脊髓及脑组织,分别测定脊髓、延髓、中脑、丘脑、海马的L-ENK含量.
结果: 造模后,模型组大鼠L-ENK含量在脊髓、中脑、海马较空白组差异均有统计学意义(P<0.01),延髓和丘脑L-ENK含量与空白组差异无统计学意义(P>0.05).针刀和电针干预后,针刀组海马中L-ENK含量较模型组升高,差异有统计学意义(P<0.05);针刀组中脑L-ENK含量较模型组降低,差异有统计学意义(P<0.05);海马中L-ENK含量针刀组较电针组高,差异有统计学意义(P<0.05);其余部位差异均无统计学意义(P>0.05).
结论: 针刀松解法对KOA大鼠中枢各水平的L-ENK有一定调节作用,其镇痛作用的发挥可能与其对中枢L-ENK的调节有关 |
| 【关键词】骨关节炎,膝 电针 脑啡呔,亮氨酸 动物实验 |
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| Affection of acupotomy lysis on Leu-Enkephalin(L-ENK) content in different parts of centrum of rats with knee osteoarthritis |
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ABSTRACT
Objective: To study the analgesia mechanism of needle-knife lysis in spinal cord and other parts of central nervous system by comparing the changes of Leu-Enkephalin (L-ENK) content in different parts of centrium of rats undergone needle-knife lysis and electro-acupuncture respectively.
Methods: Sixty healthy SD rats were randomly devided into normal control group,model group,needle-knife lysis(NKL) group and electro-acupuncture(EA) group. 4% papain solution mixed with 0.3 mol/L cysteine solutin in the ratio of 1∶1,paused for 0.5 h,injected the mixture,20 μl each time,into the left knee joint cavities of rats in model,NKL,EA groups at the 1st,4th,7th day. After 4 weeks in NKL group and EA group were treated with needle-knife lysis and electro-acupuncture,respectively. Three weeks after treatment,samples of spinal cord of the swollen part of rat waists and rat brains were taken from and the content of L-ENK of medulla oblongata,midbrain,pituitary gland,and hippocampus were measured.
Results: L-ENK content of model group increased higher than that of normal control group in spinal cord,hippocampus and midbrain (P<0.01); there were no significant difference between normal control group and modle group on L-ENK in medulla oblongata and thalamus(P>0.05). After intervening of NKL or EA,L-ENK content of NKL group increased higher in hippocampus than that of model group and EC group(P<0.05);but L-ENK content of NKL group in midbrain was lower than that of model group(P<0.05).
Conclusion: Needle-knife lysis has characteristic of regulation for the L-ENK content in different parts of central nervous system of rats with knee osteoarthritis,and analgesic effect of needle-knife was possibly related with regulation of center L-ENK. |
| KEY WORDS Osteoarthritis,knee Electroacupuncture Enkephalin,leucine Animal experimentation |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 郭长青,嵇波,陈幼楠,钟鼎文,金燕,刘清国,郭孟玮,张义,刘乃刚,陈占禄.针刀松解法对膝骨关节炎大鼠中枢不同部位亮氨酸-脑啡肽的影响[J].中国骨伤,2011,24(8):656~658 |
| 英文格式: | GUO Chang-qing,JI Bo,CHEN You-nan,ZHONG Ding-wen,JIN Yan,LIU Qing-guo,GUO Meng-wei,ZHANG Yi,LIU Nai-gang,CHEN Zhan-lu.Affection of acupotomy lysis on Leu-Enkephalin(L-ENK) content in different parts of centrum of rats with knee osteoarthritis[J].zhongguo gu shang / China J Orthop Trauma ,2011,24(8):656~658 |
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