| 大鼠马尾压迫后p75神经营养因子受体mRNA时序性表达及意义 |
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投稿时间：2011-01-07
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| 作者 | Author | 单位 | Address | E-Mail |
| 李浩鹏 |
LI Hao-peng |
西安交通大学医学院第二附属医院骨二科，陕西 西安 710004 |
The Second Department of Orthopaedics,the Second Hospital Affiliated to Medical College of Jiaotong University of Xi'an,Xi'an 710004,Shaanxi,China |
lhp-3993@163.com |
| 刘宇 |
LIU Yu |
包头医学院第一附属医院 |
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| 贺西京 |
HE Xi-jing |
西安交通大学医学院第二附属医院骨二科，陕西 西安 710004 |
The Second Department of Orthopaedics,the Second Hospital Affiliated to Medical College of Jiaotong University of Xi'an,Xi'an 710004,Shaanxi,China |
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| 徐思越 |
XU Si-yue |
西安交通大学医学院第二附属医院骨二科，陕西 西安 710004 |
The Second Department of Orthopaedics,the Second Hospital Affiliated to Medical College of Jiaotong University of Xi'an,Xi'an 710004,Shaanxi,China |
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| 陈杰 |
CHEN Jie |
西安交通大学医学院第二附属医院骨二科，陕西 西安 710004 |
The Second Department of Orthopaedics,the Second Hospital Affiliated to Medical College of Jiaotong University of Xi'an,Xi'an 710004,Shaanxi,China |
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| 冯东旭 |
FENG Dong-xu |
西安交通大学医学院第二附属医院骨二科，陕西 西安 710004 |
The Second Department of Orthopaedics,the Second Hospital Affiliated to Medical College of Jiaotong University of Xi'an,Xi'an 710004,Shaanxi,China |
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| 期刊信息：《中国骨伤》2011年，第24卷，第6期，第509-513页 |
| DOI：10.3969/j.issn.1003-0034.2011.06.021 |
| 基金项目:国家自然科学基金资助项目(编号：30672136) |
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中文摘要:
目的：观察和探讨Sprague Dawley(SD)大鼠马尾急性压迫后腰骶部脊髓p75神经营养因子受体(p75NTR)的mRNA表达和腰骶髓神经元细胞凋亡数量时间分布及关系，为马尾综合征(cauda equina syndrome,CES)发病机制提供一定理论依据。
方法：成年雌性SD大鼠60只，按时间点随机分为正常对照组及压迫1、3、5、7、14、28 d组，共计12组(n=5),造模采用L3,4节段椎管放置硅胶棒压迫70%～80%椎管面积造成马尾急性压迫症状，于术后1、3、5、7、14、28 d处置，在L1,2椎体位置脊髓取材。分别采用Tunel方法检测神经元细胞凋亡，RT-PCR方法观察p75NTR的mRNA表达。
结果：压迫组从术后1～28 d,p75 mRNA表达增加及腰骶髓神经元细胞凋亡明显，p75 mRNA表达和凋亡数目变化趋势存在先升高后降低同步趋势，均在7 d达到峰值，压迫组术后各时间点与对照组比较差异有统计学意义(P<0.05),压迫组与对照组总体差异有统计学意义(P<0.05),p75NTR的mRNA表达与腰骶部神经元细胞的凋亡呈正相关。
结论：马尾急性压迫后，p75NTR的mRNA与神经元细胞凋亡密切相关，在CES的分子发病机制中发挥重要作用。 |
| 【关键词】腰椎 神经元 脊髓 细胞凋亡 RNA,信使 |
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| p75 neurotrophin receptor mRNA sequential expression and significance after Cauda equina compression in rats |
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ABSTRACT
Objective: To observe the mRNA expression of p75NTR(p75 neurotrophin receptor)and the amount of neuronal cells apoptosis in lumbar-sacral spinal cord at different time points after the acute cauda equina compression in rats and to explore their correlation.
Methods: Sixty adult female Sprague Dawley(SD) rats were randomly divided into the normal control group and the compression groups. The acute cauda equine compression model was established as placing a silicon gel rubber at L3,4 level of the vertebral canal which represented about 70% to 80% compression to the cross section. The whole L1,2 level of spinal cords were harvested at 1,3,5,7,14,28 d after operation in compression group. Tunel method was applied to observe cell apoptosis and RT-PCR was used to detect the p75NTR mRNA expression. SPSS 13.0 statistical software was adopted to help analysis.
Results: In the compression group,both the nerve cells apoptosis and the p75 mRNA expression existed the trend of low-high-low synchronally compared with the control group,there was a significant difference (P<0.05) among comprssion groups at different time points,there was a significant difference in changes(P<0.05). p75NTR of mRNA expression and lumbosacral nerve cells apoptosis was in a positive correlation.
Conclusion: After acute cauda equina compression,p75NTR mRNA expression is closely related to the neuronal apoptosis,which plays an important role in the molecular mechanism of the CES. |
| KEY WORDS Lumbar vertebrae Neurons Spinal cord Apoptosis RNA,messenger |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 李浩鹏,刘宇,贺西京,徐思越,陈杰,冯东旭.大鼠马尾压迫后p75神经营养因子受体mRNA时序性表达及意义[J].中国骨伤,2011,24(6):509~513 |
| 英文格式: | LI Hao-peng,LIU Yu,HE Xi-jing,XU Si-yue,CHEN Jie,FENG Dong-xu.p75 neurotrophin receptor mRNA sequential expression and significance after Cauda equina compression in rats[J].zhongguo gu shang / China J Orthop Trauma ,2011,24(6):509~513 |
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