| 含自体富集骨髓干细胞松质骨移植与自体松质骨镶嵌移植修复关节软骨缺损的对比研究 |
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投稿时间：2010-08-09
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| 作者 | Author | 单位 | Address | E-Mail |
| 石仕元 |
SHI Shi-yuan |
浙江省中西医结合医院骨科, 浙江 杭州 310003 |
Department of Orthopaedics, Hospital of Integrative Medicine of Zhejiang, Hangzhou 310003, Zhejiang, China |
ssylf@xs.hz.zj.cn |
| 应小樟 |
YING Xiao-zhang |
浙江省中西医结合医院骨科, 浙江 杭州 310003 |
Department of Orthopaedics, Hospital of Integrative Medicine of Zhejiang, Hangzhou 310003, Zhejiang, China |
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| 胡德新 |
HU De-xin |
浙江省中西医结合医院骨科, 浙江 杭州 310003 |
Department of Orthopaedics, Hospital of Integrative Medicine of Zhejiang, Hangzhou 310003, Zhejiang, China |
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| 郑琦 |
ZHENG Qi |
浙江省中西医结合医院骨科, 浙江 杭州 310003 |
Department of Orthopaedics, Hospital of Integrative Medicine of Zhejiang, Hangzhou 310003, Zhejiang, China |
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| 魏威 |
WEI Wei |
浙江省中西医结合医院骨科, 浙江 杭州 310003 |
Department of Orthopaedics, Hospital of Integrative Medicine of Zhejiang, Hangzhou 310003, Zhejiang, China |
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| 期刊信息：《中国骨伤》2011年，第24卷，第4期，第332-335页 |
| DOI：10.3969/j.issn.1003-0034.2011.04.019 |
| 基金项目: |
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中文摘要:
目的：评价含富集骨髓干细胞松质骨镶嵌移植与自体松质骨镶嵌移植两种方法修复兔膝关节骨软骨缺损的修复效果, 为临床应用提供实验依据。
方法：选健康3月龄新西兰兔16只, 随机分成A、B两组, 每组8只16膝。A组采用含富集自体骨髓干细胞的自体松质骨移植, B组采用自体松质骨移植。术后第12 周处死动物取材, 分别进行大体观察、光镜观察, 并对观察指标采用Wakitani组织学评分法进行评分和秩和检验。
结果：大体观察及光镜观察显示含富集骨髓干细胞松质骨镶嵌移植组大体观察关节面大多呈乳白色, 表面比较平整, 与周围软骨融合较好, 未发现裂隙；光镜检查见关节软骨的厚度为正常软骨的2/3左右, 表面较光滑, 与周围组织接合较好, 大多为透明软骨细胞；组织学评分为(4.44±1.41)分。自体松质骨镶嵌移植组大体观察见关节面呈灰白色, 表面稍有凹陷, 欠光整；光镜检查见关节软骨的厚度较薄, 为正常软骨的1/3～1/2, 移植物中央区域厚度更薄, 见成纤维细胞和极少量透明软骨细胞；组织学评分为(8.93±1.18)分。两组组织学评分比较差异有统计学意义(P<0.01).
结论：含富集骨髓干细胞松质骨镶嵌移植能以类透明软骨组织修复全层关节软骨缺损, 可用于较大面积软骨缺损的修复。自体松质骨移植修复软骨缺损的能力较差。 |
| 【关键词】膝关节 软骨，关节 骨移植 创伤和损伤 |
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| Experiment on articular cartilage defect repaired with autologous cancellous bone or cancellous bone enriching bone marrow stem cell |
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ABSTRACT
Objective: To compare and evaluate the effect of autologous cancellous bone and cancellous bone enriching bone marrow stem cell for the repairing the defects of articular cartilage, and purpose the experimental basis for clinical application.
Methods: By using the completely random design, 16 adult rabbits were divided into two groups randomly. Autologous cancellous bone and cancellous bone enriching bone marrow stem cells were applied for repairing size-matched, full-thickness articular cartilage defects on the femoral condyle of the knees. The reconstructed tissues were observed by gross, optical and microscopy view and Wakitani score at 12 weeks respectively.
Results: In cancellous bone enriching bone marrow stem cells group, articular surface was ivory white and relative evenness, the regenerated tissues integrated well with the surrounding normal cartilage with obscure boundary between them. The thickness of regenerated tissues was two-third of normal cartilage, the Wakitani score was 4.44±1.41. In autologous cancellous bone group, articular surface was gray and introcession, the regenerated tissues was very thin, the thickness of regenerated tissues was one thirds or one half of normal cartilage, the Wakitani score was 8.93±1.18. The differences between two groups were significant(P<0.01).
Conclusion: Cancellous bone enriching bone marrow stem cells are feasible for repairing of large articular cartilage defects with hyaline cartilage. The repairing ability of autologous cancellous bone is inferior. |
| KEY WORDS Knee joint Cartilage, articular Bone transplantation Wounds and injuries |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 石仕元,应小樟,胡德新,郑琦,魏威.含自体富集骨髓干细胞松质骨移植与自体松质骨镶嵌移植修复关节软骨缺损的对比研究[J].中国骨伤,2011,24(4):332~335 |
| 英文格式: | SHI Shi-yuan,YING Xiao-zhang,HU De-xin,ZHENG Qi,WEI Wei.Experiment on articular cartilage defect repaired with autologous cancellous bone or cancellous bone enriching bone marrow stem cell[J].zhongguo gu shang / China J Orthop Trauma ,2011,24(4):332~335 |
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