| FK506缓释剂促进周围神经再生纤维电生理功能恢复的实验研究 |
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投稿时间：2010-07-06
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| 作者 | Author | 单位 | Address | E-Mail |
| 李强 |
LI Qiang |
解放军第98医院全军创伤骨科中心 浙江 湖州 313000 |
Center of Micro-Orthopaedics of Nanjing Military, 98th Hospital, Hu Zhou 313000, Zhejiang, China |
lqlq101@126.com |
| 申屠刚 |
SHEN Tu-gang |
解放军第98医院全军创伤骨科中心 浙江 湖州 313000 |
Center of Micro-Orthopaedics of Nanjing Military, 98th Hospital, Hu Zhou 313000, Zhejiang, China |
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| 伍亚民 |
WU Ya-min |
第三军医大学创伤外科研究所 |
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| 李骥 |
LI Ji |
解放军第98医院全军创伤骨科中心 浙江 湖州 313000 |
Center of Micro-Orthopaedics of Nanjing Military, 98th Hospital, Hu Zhou 313000, Zhejiang, China |
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| 王刚 |
WANG Gang |
解放军第98医院全军创伤骨科中心 浙江 湖州 313000 |
Center of Micro-Orthopaedics of Nanjing Military, 98th Hospital, Hu Zhou 313000, Zhejiang, China |
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| 期刊信息：《中国骨伤》2010年，第23卷，第11期，第841-844页 |
| DOI：10.3969/j.issn.1003-0034.2010.11.014 |
| 基金项目: |
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中文摘要:
目的：探讨FK506缓释剂促进周围神经再生的效果。
方法：2008年3月至9月，选取32只SD大鼠，雌雄不拘，体重200～250 g,1%戊巴比妥钠(30 mg/kg体重)腹腔内注射麻醉。取股后斜切口，暴露右侧坐骨神经，10倍显微镜下游离坐骨神经，并在梨状肌出口下方5 mm处将坐骨神经切除约8 mm,任其回缩，造成10 mm缺损，用梭形双通道桥接管桥接坐骨神经10 mm缺损，根据梭形管的两支管内加入的药物不同将动物随机均分为A组(两支管内均加入几丁糖凝胶)和B组[一侧支管内注入几丁糖加生理盐水(B1组);另一侧支管内注入几丁糖加 FK506(B2组)].每组16只，于术后8、12周对再生神经的组织病理学进行观察，并对术后12周的再生神经行皮层感觉诱发电位(CSEP)、复合肌肉动作电位(CMAP)及神经传导速度(CV)进行测定。
结果：术后8、12周，A组两支管内再生神经纤维组织病理形态无明显差异，电生理功能检测无统计学意义；B组注入FK506 侧(B2组)再生纤维粗细均匀，排列整齐，明显优于对照侧(B1组);术后12周，B2组较B1组的CSEP、CMAP的潜伏期短，而振幅高，传导速度快。两者有统计学差异(P<0.01).
结论：FK506可明显促进周围神经再生纤维电生理功能恢复，为周围神经损伤免疫抑制剂提供理论依据。 |
| 【关键词】周围神经 电生理学 免疫法 |
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| Experimental study of electrophysiologic effects of regenerative nerve fibres affected by control releasing FK506 |
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ABSTRACT
Objective: To discuss the electrophysiologic effects of regenerative nerve fibres affected by control releasing of FK506.
Methods: From Mar. to Sep. in 2008,the body weigh of 32 Sprague-Dawley rats which was 200 to 250 g,anesthesia was performed with an intraperitoneal injection of 30 mg/kg 1% continal. The sciatic nerve was transected in each rat by the excision of a 10 mm gap just proximal to the trifurcation of the nerve. The 10 mm gap of sciatic nerve had been bridged with the new double channel nerve conduit of fusiform shape,which were randomly divided into two groups basing on the different drug in the channel,each group contained 16 animals. In group A,100 μl of chitin for medical use was injected into the conduit,in group B the two branches of the conduit respectively contained 100 μl of the chitin and 10 μl FK506(group B2) or physiologic saline(group B1). At 8 and 12 week after operation,the morphology in regenerative nerve and electrophysiologic effects by detect compound muscle active potential(CMAP) and cortical somatosensory evoked potential(CSEP) were evaluated.
Results: There were not significant differences of the regenerative nerve fibres between two channels in group A,but in group B2,the number of the regenerative fibres was much more than that in group B1. The latency of CMAP and CSEP in group B2 was shorter than that in group B1. But its amplitude was higher. There were highly significant difference between the groups(P<0.01).
Conclusion: The electrophysiologic effects of regenerative nerve fibres can be significantly promoted by FK506,which provide theory base for immunosuppressive treatment of peripheral nerve. |
| KEY WORDS Peripheral nerves Electrophysiology Immunization |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 李强,申屠刚,伍亚民,李骥,王刚.FK506缓释剂促进周围神经再生纤维电生理功能恢复的实验研究[J].中国骨伤,2010,23(11):841~844 |
| 英文格式: | LI Qiang,SHEN Tu-gang,WU Ya-min,LI Ji,WANG Gang.Experimental study of electrophysiologic effects of regenerative nerve fibres affected by control releasing FK506[J].zhongguo gu shang / China J Orthop Trauma ,2010,23(11):841~844 |
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