| Ilizarov技术对关节软骨病理改变的实验观察 |
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投稿时间：2009-10-13
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| 作者 | Author | 单位 | Address | E-Mail |
| 韩大为 |
HAN Da-wei |
福建中医学院附属人民医院骨科,福建 福州 350004 |
Department of Orthopaedics,the People's Hospital Affiliated to Fujian College of TCM,Fuzhou 350004,Fujian,China |
handawei@medmail.com.cn |
| 李良华 |
LI Liang-hua |
福建中医学院附属人民医院骨科,福建 福州 350005 |
Department of Orthopaedics,the People's Hospital Affiliated to Fujian College of TCM,Fuzhou 350005,Fujian,China |
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| 王清玉 |
WANG Qing-yu |
福建中医学院附属人民医院骨科,福建 福州 350006 |
Department of Orthopaedics,the People's Hospital Affiliated to Fujian College of TCM,Fuzhou 350006,Fujian,China |
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| 卢圣友 |
LU Sheng-you |
福建中医学院附属人民医院骨科,福建 福州 350007 |
Department of Orthopaedics,the People's Hospital Affiliated to Fujian College of TCM,Fuzhou 350007,Fujian,China |
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| 期刊信息：《中国骨伤》2009年，第22卷，第12期，第923-926页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:自然科学基金高校专项(编号：2006J0426) |
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中文摘要:
目的：了解在Ilizarov技术下，受牵拉的实验踝关节软骨之间的压力改变对关节软骨的影响，为临床提供理论依据。
方法：在18只实验狗左后足踝关节处分别装上Ilizarov外固定支架，采用1 mm/d的牵拉速度，先制造内翻畸形，时间3周，致踝关节内翻15°，摄X线片证实后，停止牵拉3周，再以相同的速度(1 mm/d)矫正畸形，时间3周。并于第3、7、9周(分组为：A组、B组、C组)取实验足内侧关节软骨和健足内侧关节软骨(D组),通过HE染色，观察内侧关节软骨形态学改变。通过免疫组织化学染色，应用软件Image-Pro Plus 6.0取得图像灰度值，用SPSS 13.0进行统计学处理。使用透射电镜观察软骨的显微结构。
结果：① Mankin得分：手术后A组和B组Mankin得分差异有统计学意义(P=0.043<0.05);手术后B组和C组Mankin得分差异有统计学意义(P=0.038<0.05).②灰度值：A组和B组比较，P=0.047<0.05,差异有统计学意义；B组和C组比较，P=0.045<0.05,差异有统计学意义；A组和D组比较，P=0.039<0.05,差异有统计学意义；C组和D组比较，P=1.23>0.05,差异无统计学意义。
结论：在Ilizarov牵张技术牵拉下，当踝关节软骨面之间压力增大时，关节软骨会发生压力性坏死；当关节软骨面之间压力逐渐减小后，关节软骨可逐渐恢复再生。 |
| 【关键词】踝关节 软骨 骨再生 病理学 |
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| Effects of Ilizarov technique on the histopathological changes of articular cartilage |
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ABSTRACT
Objective: To study the effects of pressure changes on the experimental articular cartilage by utilizing Ilizarov distraction technique.
Methods: The Ilizarov external fixators were fixed at the left ankle joints of 18 experimental dogs,and the fixators were stretched with a speed of 1 mm /d to create a varus deformity. After three weeks,the ankle varus was 15 degree;when the degree was confirmed by X-ray film,the same traction was retained for 3 weeks. Then the traction decreased at the same speed of 1 mm /d to correct the deformity for 3 weeks. At the 3rd,7th and 9th weeks,articular cartilages were separated from medial foot of the experimental and normal side to do morphology observation after HE stain,which were group A,group B,group C and group D respectively separately. The gray scale values were obtained using Image-Pro Plus 6.0 software and compared among the 4 groups using SPSS 13.0. The microstructure of cartilage was observed through transmission electron microscopy.
Results: ① The Mankin scores: there was significant difference between group A and group B(P=0.043<0.05); and significant difference between group B and group C(P=0.038<0.05). ② The values of gray scale: there was significant difference between group A and group B(P=0.047<0.05); significant difference between group B and group C(P=0.045<0.05);significant difference between group A and group D(P=0.039<0.05);no significant difference between group C and group D(P=1.23>0.05).
Conclusion: Using Ilizarov technique,when the pressure between the cartilage surface of the ankle joint increases,the necrosis of articular cartilage occurred;and when the stress between the articular cartilage surface decreases,the damaged articular cartilage can be restored. |
| KEY WORDS Ankle joint Cartilage,articular Bone regeneration Pathology |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 韩大为,李良华,王清玉,卢圣友.Ilizarov技术对关节软骨病理改变的实验观察[J].中国骨伤,2009,22(12):923~926 |
| 英文格式: | HAN Da-wei,LI Liang-hua,WANG Qing-yu,LU Sheng-you.Effects of Ilizarov technique on the histopathological changes of articular cartilage[J].zhongguo gu shang / China J Orthop Trauma ,2009,22(12):923~926 |
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