| 骨关节炎软骨细胞中结缔组织生长因子与巨噬
细胞集落刺激因子和软骨退变相关性的研究 |
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投稿时间：2009-02-03
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| 作者 | Author | 单位 | Address | E-Mail |
| 孙剑 |
SUN Jian |
上海中医药大学附属市中医医院骨伤科,上海 200071 |
Department of Orthopaedics and Traumatology,Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of TCM,Shanghai 200071,China |
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| 陈朝蔚 |
CHEN Chao-wei |
上海中医药大学附属市中医医院骨伤科,上海 200071 |
Department of Orthopaedics and Traumatology,Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of TCM,Shanghai 200071,China |
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| 何永淮 |
HE Yong-huai |
上海中医药大学附属市中医医院骨伤科,上海 200071 |
Department of Orthopaedics and Traumatology,Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of TCM,Shanghai 200071,China |
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| 裘敏蕾 |
QIU Min-lei |
上海中医药大学附属市中医医院骨伤科,上海 200071 |
Department of Orthopaedics and Traumatology,Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of TCM,Shanghai 200071,China |
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| 陈永强 |
CHEN Yong-qiang |
上海中医药大学附属市中医医院骨伤科,上海 200071 |
Department of Orthopaedics and Traumatology,Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of TCM,Shanghai 200071,China |
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| 期刊信息：《中国骨伤》2009年，第22卷，第6期，第451-453页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:上海市基础研究重点项目资助(编号：08JC1418800) |
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中文摘要:
目的： 观察结缔组织生长因子(connective tissue growth factor,CTGF)在骨关节炎软骨细胞中的表达及其与巨噬细胞集落刺激因子(macrophage colony-stimulating factor,M-CSF)和关节炎软骨退变过程中Mankin评分的关系， 探讨骨关节炎软骨细胞中CTGF与M-CSF、骨关节炎软骨退变相关性。
方法：新西兰大白兔10只随机分为正常组、模型组。按照Hulth法建立膝骨关节炎模型，于术后第8周处死，取各组胫骨平台关节软骨，采用番红O染色，进行Mankin评分。免疫组织化学染色测定关节软骨中CTGF与M-CSF表达的阳性指数，应用Spearman相关分析检验CTGF分别与M-CSF和Mankin评分结果之间有无相关性。
结果：模型组CTGF、M-CSF染色阳性指数与正常组比较增高，其差异有统计学意义(P<0.05).统计学分析结果显示，CTGF与Mankin评分间呈正相关(r=0.542,P=0.001);CTGF与M-CSF的阳性表达的相关性分析结果显示，软骨细胞中CTGF与M-CSF的表达呈正相关(r=0.634,P=0.007).
结论：骨关节炎中CTGF和M-CSF表达密切相关，M-CSF表达增强可能是促进CTGF表达增强的重要途径之一，CTGF与M-CSF蛋白表达的上调在骨关节炎软骨退变中起重要作用。 |
| 【关键词】骨关节炎 软骨细胞 巨噬细胞集落刺激因子 结缔组织生长因子 |
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| Study on correlation between connective tissue growth factor, macrophage colony-stimulating factor and cartilage degeneration in the osteoarthritis chondrocytes |
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ABSTRACT
Objective: To investigate the expression of connective tissue growth factor(CTGF) in osteoarthritis chondrocytes,and the relationship between CTGF,macrophage colony-stimulating factor(M-CSF) and cartilage degeneration.
Methods: Ten New Zealand white rabbits were randomly divided into the normal group and OA model group established by Hulth's method. Sections were stained with Safranin O for histological examination. The cartilage histological characteristics were observed according to the method of Mankin. Immunohistochemical staining was performed. Articular cartilages were observed with microscopy and the image analysis method was used to measure the expression intensity of CTGF and M-CSF in each group,and the correlations of the expression of CTGF,M-CSF and cartilage degeneration were analyzed by statistics.
Results: Immunohistochemical staining indicated that the expression intensity of CTGF,M-CSF in untreated group was significantly increased as compared with that in the normal group(P<0.05). Statistical analysis showed that there was a correlation between the expression of CTGF,M-CSF and cartilage degeneration(r=0.634,r=0.542,P<0.01 respectively).
Conclusion: The expression of CTGF and M-CSF protein is up regulated in osteoarthritis chondrocytes,which suggests that the activation of M-CSF is involved in the production of CTGF. CTGF and M-CSF play an important role in the pathogenesis of cartilage degeneration. |
| KEY WORDS Osteoarthritis Chondrocytes Macrophage colony-stimulating factor Connective tissue growth factor |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 孙剑,陈朝蔚,何永淮,裘敏蕾,陈永强.骨关节炎软骨细胞中结缔组织生长因子与巨噬
细胞集落刺激因子和软骨退变相关性的研究[J].中国骨伤,2009,22(6):451~453 |
| 英文格式: | SUN Jian,CHEN Chao-wei,HE Yong-huai,QIU Min-lei,CHEN Yong-qiang.Study on correlation between connective tissue growth factor, macrophage colony-stimulating factor and cartilage degeneration in the osteoarthritis chondrocytes[J].zhongguo gu shang / China J Orthop Trauma ,2009,22(6):451~453 |
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