| 淫羊藿总黄酮对去卵巢大鼠骨组织OPG、
OPGL mRNA表达的影响 |
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投稿时间：2008-12-10
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| 作者 | Author | 单位 | Address | E-Mail |
| 陈柏龄 |
CHEN Bai-ling |
中山大学附属第一医院骨科,广东 广州 510080 |
Department of Orthopaedics,the First Affiliated Hospital of SUN Yat-sen University,Guangzhou 510080,Guangdong,China |
berlinch2007@yahoo.com.cn |
| 谢登辉 |
XIE Deng-hui |
中山大学附属第一医院骨科,广东 广州 510080 |
Department of Orthopaedics,the First Affiliated Hospital of SUN Yat-sen University,Guangzhou 510080,Guangdong,China |
|
| 王宗伟 |
WANG Zong-wei |
广州中医药大学中药学院 |
|
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| 李佛保 |
LI Fo-bao |
中山大学附属第一医院骨科,广东 广州 510080 |
Department of Orthopaedics,the First Affiliated Hospital of SUN Yat-sen University,Guangzhou 510080,Guangdong,China |
|
| 徐栋梁 |
XU Dong-liang |
中山大学附属第一医院骨科,广东 广州 510080 |
Department of Orthopaedics,the First Affiliated Hospital of SUN Yat-sen University,Guangzhou 510080,Guangdong,China |
|
| 黎艺强 |
LI Yi-qiang |
中山大学附属第一医院骨科,广东 广州 510080 |
Department of Orthopaedics,the First Affiliated Hospital of SUN Yat-sen University,Guangzhou 510080,Guangdong,China |
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| 期刊信息：《中国骨伤》2009年，第22卷，第4期，第271-273页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:广东省中医药局基金资助(编号：1040059) |
|
中文摘要:
目的：探讨淫羊藿总黄酮(total flavone of epimedium,TFE)治疗骨质疏松症的分子机制，为传统中药的现代化和二次开发提供实验依据。
方法：将60只4月龄健康雌性SD大鼠按随机数字表法分为对照组(A组，20只，行假手术处理),去卵巢组(OVX组，20只，切除卵巢后不给予淫羊藿处理),淫羊藿组(TFE组，20只，切除卵巢后给予淫羊藿灌胃).所有大鼠术前及术后4周以DEXA骨密度仪检测L4骨密度变化(若BMD下降>20%,则骨质疏松模型建立).模型建立后TFE组大鼠给予淫羊藿总黄酮(浓度30 mg/ml,10 ml/kg,1次/d)灌胃4周。所有大鼠处死前再行DEXA骨密度检测，过量麻醉法处死后取其股骨下部，切片匀浆提取骨组织中RNA,应用逆转录-聚合酶链反应(RT-PCR)技术检测骨组织护骨素(osteoprotegrin,OPG)、护骨素配体(OPGL) mRNA的表达。
结果：①TFE组大鼠切除卵巢4周后，腰椎BMD均值降至(0.084±0.020) g/cm2,降幅>20%证明骨质疏松模型建立。TFE灌胃4周后其腰椎BMD提高至(0.112±0.009) g/cm2,与给药前比较有明显改善，差异有统计学意义(P<0.05); ②TFE组大鼠骨组织中OPG mRNA 的表达较OVX组相比，明显增强，且有统计学差异(P<0.05),但对OPGL mRNA表达促进作用不明显，组间无统计学差异(P>0.05).
结论：TFE是通过促进骨组织中OPG mRNA的表达来抑制破骨细胞的分化和成熟，从而达到治疗骨质疏松症的目的。 |
| 【关键词】淫羊藿 骨质疏松 护骨素 动物实验 |
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| Effect of total flavone of epimedium on expression of bone OPG,OPGL mRNA in ovariectomized rats |
|
ABSTRACT
Objective: To investigate the molecular mechanism of TFE(total flavone of epimedium) in the treatment of osteoporosis,and then provide experimental evidence for modernization and further development of TFE as an traditional Chinese medicine.
Methods: Sixty healthy female SD rats with aged 4 months were randomly divided into three groups (including control group in which rats received sham surgery,OVX group in which ovariectomized rats didn't give any medicine after the removal of ovaries and TFE group in which ovariectomized rats administrated TFE),20 rats in each group. Compared bone mineral density(BMD) between before operation and at 4th week after operation in order to verify the establishment of osteoporotic model(criteria:BMD decreased more than 20% at 4th week after operation). The rats in TEF group were administrated total flavone of epimedium(concentration 30 mg/ml,10 ml/kg,qd) orally for 4 weeks. After this,killed rats to harvest the lower part of the femur and detected BMD again. Applying the reverse transcriptase-polymerase chain reaction technique (RT-PCR) to detect expression of OPG,OPGL mRNA in bone tissue.
Results: ①At 4th week after ovariectomy,the mean BMD of lumbar vertebra in TFE group fell to(0.084 ± 0.020) g/cm2. Administrated with TFE for 4 weeks,the BMD increased to(0.112 ± 0.009) g/cm2. There was significant improvement compare with the OVX group(P<0.05). ②Compared between OVX group and TFE group,The OPG mRNA expression of TFE group obviously enhanced. There was significant difference in statistics(P<0.05). However,the promotion for OPGL mRNA expression were detected between OVX group and TFE group,there was no significant difference in statistics(P>0.05).
Conclusion: This study showed that TFE could inhibit differentiation and maturation of osteoclast through enhancing OPG mRNA expression,accordingly,to treat osteoporosis. |
| KEY WORDS Epimedium Osteoporosis Osteoprotegerin Animal experimentation |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 陈柏龄,谢登辉,王宗伟,李佛保,徐栋梁,黎艺强.淫羊藿总黄酮对去卵巢大鼠骨组织OPG、
OPGL mRNA表达的影响[J].中国骨伤,2009,22(4):271~273 |
| 英文格式: | CHEN Bai-ling,XIE Deng-hui,WANG Zong-wei,LI Fo-bao,XU Dong-liang,LI Yi-qiang.Effect of total flavone of epimedium on expression of bone OPG,OPGL mRNA in ovariectomized rats[J].zhongguo gu shang / China J Orthop Trauma ,2009,22(4):271~273 |
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