| 成骨生长肽对大鼠骨髓基质细胞增殖和成骨分化的作用 |
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投稿时间：2008-04-16
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| 作者 | Author | 单位 | Address | E-Mail |
| 肖毅 |
XIAO Yi |
山东大学生命科学学院发育免疫学实验室，山东 济南 250100 |
Developmental Immunology Lab,School of Life Science,Shandong University,Jinan 250100,Shandong,China |
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| 王建国 |
WANG Jian-guo |
山东大学生命科学学院发育免疫学实验室，山东 济南 250100 |
Developmental Immunology Lab,School of Life Science,Shandong University,Jinan 250100,Shandong,China |
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| 白增亮 |
BAI Zeng-liang |
山东大学生命科学学院发育免疫学实验室，山东 济南 250100 |
Developmental Immunology Lab,School of Life Science,Shandong University,Jinan 250100,Shandong,China |
zengliangbai@mail.sdu.edu.cn |
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| 期刊信息：《中国骨伤》2008年，第21卷，第11期，第843-845页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:山东省博士后基金(编号：11200004040740) |
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中文摘要:
目的：观察成骨生长肽诱导体外培养的大鼠骨髓基质细胞增殖及向成骨分化的作用。
方法：取6周龄SD大鼠，贴壁法分离培养骨髓基质细胞，在不同浓度成骨生长肽的诱导下，观察细胞形态变化，绘制骨髓基质细胞生长曲线，碱性磷酸酶和钙结节组织化学染色。
结果：成骨生长肽对骨髓基质细胞增殖和成骨分化的作用呈剂量依赖性：成骨生长肽浓度在10-10 及10-11 mol/L时促进骨髓基质细胞增殖，而在10-8及10-9 mol/L时对骨髓基质细胞的增殖略有抑制作用；成骨生长肽浓度在10-10 及10-11 mol/L时骨髓基质细胞碱性磷酸酶染色基本呈阴性，与对照组相比差异无统计学意义，而在10-8 及10-9 mol/L浓度下可以显着提高骨髓基质细胞碱性磷酸酶染色的阳性率。
结论：成骨生长肽可以明显促进大鼠骨髓基质细胞增殖及向成骨细胞分化，其促成骨活性具有显着的浓度依赖性。 |
| 【关键词】成骨生长肽 骨髓基质细胞 细胞分化 |
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| Effects of osteogenic growth peptide to the proliferation and differentiation of bone marrow stromal cells |
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ABSTRACT
Objective: To investigate the effects of osteogenic growth peptide(OGP) to the proliferation and differentiation of cultured bone marrow stromal cells (BMSCs) of rats.
Methods: The SD rats(age 6 weeks) were sacrified,and the bone marrow stromal cells as the adherent stromal cell population were separated from the bone marrow culcure. The proliferation curves of bone marrow stromal cells which were conditioned cultured with four kind of different concentrations of osteogenic growth peptide were measured with the MTT method,and the osteogenesis markers including alkaline phosphatase and calcic deposition detected by histochemical staining.
Results: Osteogenic growth peptide at the concentration of 10-10,10-11 mol/L promoted the proliferation of bone marrow stromal cells,while at the concentrations of 10-8,10-9 mol/L suppressed the proliferation of bone marrow stromal cells. However,osteogenic growth peptide at the concentration of 10-9,10-8 mol/L advanced the ratio of positive cells in alkaline phosphatase histochemical staining.
Conclusion: Osteogenic growth peptide shows distinct effects on the proliferation and differentiation of bone marrow stromal cells depending on its concentration. Osteogenic growth peptide at the concentration of 10-8,10-9 mol/L can promote bone marrow stromal cell differentiation to the osteogenic in vitro. |
| KEY WORDS Osteogenic growth peptide Bone marrow stromal cells Cell differentiation |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 肖毅,王建国,白增亮.成骨生长肽对大鼠骨髓基质细胞增殖和成骨分化的作用[J].中国骨伤,2008,21(11):843~845 |
| 英文格式: | XIAO Yi,WANG Jian-guo,BAI Zeng-liang.Effects of osteogenic growth peptide to the proliferation and differentiation of bone marrow stromal cells[J].zhongguo gu shang / China J Orthop Trauma ,2008,21(11):843~845 |
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