| 神经干细胞移植促进脊髓损伤后脑源性神经营养因子的表达 |
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投稿时间：2008-06-05
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| 作者 | Author | 单位 | Address | E-Mail |
| 王岩峰 |
WANG Yan-feng |
中国医科大学附属第一医院骨科， 辽宁 沈阳 110001 |
Department of Orthopaedics,the first Affiliated Hospital,China Medical University,Shenyang 110001,Liaoning,China |
wyf_doctor@163.com |
| 吕刚 |
LV Gang |
辽宁医学院 |
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| 赵宇 |
ZHAO Yu |
中国医科大学附属第一医院骨科， 辽宁 沈阳 110001 |
Department of Orthopaedics,the first Affiliated Hospital,China Medical University,Shenyang 110001,Liaoning,China |
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| 金哲 |
JIN Zhe |
中国医科大学附属第一医院骨科， 辽宁 沈阳 110001 |
Department of Orthopaedics,the first Affiliated Hospital,China Medical University,Shenyang 110001,Liaoning,China |
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| 黄涛 |
HUANG Tao |
中国医科大学附属第一医院骨科， 辽宁 沈阳 110001 |
Department of Orthopaedics,the first Affiliated Hospital,China Medical University,Shenyang 110001,Liaoning,China |
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| 于德水 |
YU De-shui |
中国医科大学附属第一医院骨科， 辽宁 沈阳 110001 |
Department of Orthopaedics,the first Affiliated Hospital,China Medical University,Shenyang 110001,Liaoning,China |
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| 董宝铁 |
DONG Bao-tie |
中国医科大学附属第一医院骨科， 辽宁 沈阳 110001 |
Department of Orthopaedics,the first Affiliated Hospital,China Medical University,Shenyang 110001,Liaoning,China |
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| 期刊信息：《中国骨伤》2008年，第21卷，第11期，第836-838页 |
| DOI：doi:10.3969/j.issn.1003-0034.yyyy.nn.zzz |
| 基金项目:1.教育部高校博士点专项科研基金(编号：20060159019);2.辽宁省自然科学基金项目(编号：20052096) |
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中文摘要:
目的：探讨神经干细胞(NSCs)移植对大鼠脊髓损伤后脑源性神经营养因子(BDNF)表达的影响及其意义。
方法：NSCs提取自新生Wistar大鼠的海马区，经培养、鉴定。制作大鼠脊髓损伤(SCI)模型，于伤后第7天移植NSCs.实验分为3组：NSCs移植组(A组),DMEM填充组(B组),正常对照组(C组).应用RT-PCR法和免疫组化法观察细胞移植后BDNF基因表达的变化。
结果：RT-PCR结果分析，移植术后第1、3、5天，A组BDNF mRNA的表达量明显高于B组，差异有统计学意义(P<0.05).组化结果分析，移植术后第7、14、28天BDNF的表达量A组明显高于B组，差异有统计学意义(P<0.05).
结论：NSCs在移植后可上调脑源性神经营养因子BDNF基因的表达，是其修复脊髓损伤的机制之一。 |
| 【关键词】脊髓损伤 神经干细胞 脑源性神经营养因子 |
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| Neural stem cells transplantation promote the expressions of brain derived neurotrophic factor after the spinal cord injury of rats |
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ABSTRACT
Objective: To observe the effects of neural stem cells (NSCs) transplantation on the brain derived neurotrophic factor(BDNF) after the spinal cord injury(SCI) of rats,and to investigate the mechanism of repairing the SCI by NSCs transplantation.
Methods: Neural stem cells were cultured from the hippocampus of rats' embryo and identified by immunocytochemistry. Seven days after the operation of SCI,the NSCs were transplanted into the injured site. Sixty adult Wistar rats were randomly divided into three groups:SCI cured with NSCs transplantation (group A),SCI received DMEM solution (group B), control group(group C). Then the expression of BDNF of the lesion and neighbor areas were examined by reverse transcsription polymerase chain reaction(RT-PCR) and immunohistochemistry,so as to investigated the mechanism of repairing the SCI after NSCS transplantation.
Results: According the RT-PCR results analysis,the expression of BDNF mRNA of group A enhanced higher than that of group B on the 1st,3rd,5th day after transplantation of NSCs. According the immunohistochemistry results analysis,the expression of BDNF mRNA of group A enhanced higher than that of group B on the 7 th,14 th,28 th day similarly.
Conclusion: The transplantation of NSCs can change the tiny-entironment by upregulating the expression of BDNF. It maybe one of the mechanism of repairing the SCI by NSCs transplantation. |
| KEY WORDS Spinal cord injuries Neural stem cells Brain-derived neurotrophic factor |
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| 引用本文，请按以下格式著录参考文献： |
| 中文格式: | 王岩峰,吕刚,赵宇,金哲,黄涛,于德水,董宝铁.神经干细胞移植促进脊髓损伤后脑源性神经营养因子的表达[J].中国骨伤,2008,21(11):836~838 |
| 英文格式: | WANG Yan-feng,LV Gang,ZHAO Yu,JIN Zhe,HUANG Tao,YU De-shui,DONG Bao-tie.Neural stem cells transplantation promote the expressions of brain derived neurotrophic factor after the spinal cord injury of rats[J].zhongguo gu shang / China J Orthop Trauma ,2008,21(11):836~838 |
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